Exonuclease 1 Antibody - BSA Free

Western Blot: Exonuclease 1 Antibody [NBP2-16391] - Sample (50 ug of whole cell lysate) A: mouse thymus, 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Exonuclease 1 Antibody [NBP2-16391] - Analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody (NBP2-16391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Exonuclease 1 antibody detects Exonuclease 1 protein at nucleus by immunofluorescent analysis.Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Exonuclease 1 protein stained by Exonuclease 1 antibody (NBP2-16391) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining.

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Exonuclease 1 antibody (NBP2-16391) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Exonuclease 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Exonuclease 1 Antibody - BSA Free (NBP2-16391) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-Exonuclease 1 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Exonuclease 1. The exact sequence is proprietary.
• Localization: Nucleus
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EXO1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Exonuclease 1 Antibody - BSA Free

• EXOI
• exonuclease 1
• Exonuclease I
• HEX1rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
• hExo1
• hExoIEC 3.1

Background

This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Exonuclease 1 Antibody (NBP2-16391)(5)

Publications using NBP2-16391

• Nusawardhana, A;Pale, LM;Nicolae, CM;Moldovan, GL; USP1-dependent nucleolytic expansion of PRIMPOL-generated nascent DNA strand discontinuities during replication stress Nucleic acids research 2024-01-05 [PMID: 38180818]
• Zhao L, Zang Q, Liang G, Yao X LncRNA CECR7 boosts hepatocellular carcinoma progression by recruiting RNA binding protein U2AF2 to enhance the stability of EXO1 mRNA Heliyon 2023-09-01 [PMID: 37809785] (WB, Human)
• Hale A, Dhoonmoon A, Straka J et al. Multi-step processing of replication stress-derived nascent strand DNA gaps by MRE11 and EXO1 nucleases Nature communications 2023-10-07 [PMID: 37805499] (PLA)
• Dhoonmoon A, Nicolae CM, Moldovan GL The KU-PARP14 axis differentially regulates DNA resection at stalled replication forks by MRE11 and EXO1 Nature communications 2022-08-27 [PMID: 36030235] (KD, WB, Human)
  
  Details:
  Supplementary Figure S1. H: Western blots confirming knockdowns of ZRANB3 and PARP10 in HeLa-BRCA2KO cells.
• Xu X, Ni K, He Y et al. The epigenetic regulator LSH maintains fork protection and genomic stability via MacroH2A deposition and RAD51 filament formation Nature communications 2021-06-10 [PMID: 34112784] (WB, Human)

Bioinformatics

• Gene Symbol: EXO1
• Uniprot:
  • Q9UQ84(Human)