PMP22 Antibody (Hu1) - Azide and BSA Free

Immunohistochemistry: PMP22 Antibody (Hu1) - Azide and BSA Free [NBP2-80913] - Staining of PMP22 (brown) in dorsal root ganglion and spinal roots in 20 week fetal spinal cord preparation from rhesus monkey. Image from the standard format of this antibody.

• Reactivity: Hu, Pm, Bv(-), Mu(-), Rt(-)
• Applications: WB, IHC
• Clone: Hu1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.9 mg/ml

PMP22 Antibody (Hu1) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PMP22 Antibody (Hu1) - Azide and BSA Free (NB110-59086) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human PMP22 cDNA boosted with a 13-amino acid peptide of the 2nd extracellular domain (a.a. 120-133) conjugated to murine IgG. [Swiss-Prot# Q01453]
• Epitope: Detects amino acids 121-123 in the second extracellular domain of human PMP22.
• Localization: Membrane
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PMP22
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot reported in scientific literature

Reactivity Notes

Rhesus monkey. Does not react with mouse, rat, or bovine nerve protein.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 0.9 mg/ml
• Purity: Protein G purified

Alternate Names for PMP22 Antibody (Hu1) - Azide and BSA Free

• CMT1A
• CMT1E
• GAS3
• GAS-3DSS
• growth arrest-specific 3
• Growth arrest-specific protein 3
• HMSNIA
• HNPP
• MGC20769
• peripheral myelin protein 22
• PMP-22
• Sp110

Background

PMP22 (peripheral myelin protein-22) is an integral membrane glycoprotein of internodal myelin and comprises an estimated 2-5 % of total myelin protein contents of peripheral nervous system. Its functional importance is emphasized by the fact that PMP22 gene mutations are the most common causes for inherited peripheral nerve disorders, also known as CMT (Charcot-Marie-Tooth diseases). Mutations of PMP22 are responsible for more than half of all the patients with inherited peripheral neuropathies, including CMT1A/CMT- type-1A with trisomy of PMP22, hereditary neuropathy with liability to pressure palsies (HNPP) with heterozygous deletion of PMP22, and CMT1E with point mutations of PMP22. These PMP22 associated disorders disrupt the organization of myelin, and subsequently axonal integrity which is untimately responsible for disabilities in patients. While overexpression and point-mutations of the PMP22 gene produces gain-of-function phenotypes, PMP22 deletion results in a loss-of-function phenotype that reveals the normal physiological functions of the PMP22 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PMP22