PLK1 Antibody (OTI1D4) - Azide and BSA Free

Western Blot: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.

Flow Cytometry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control plasmid (Blue) were immunostaining by anti-PLK1 antibody , and then analyzed by flow cytometry

Western Blot: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.

Immunohistochemistry: PLK1 Antibody (OTI1D4) - Azide and BSA Free [NBP2-73472] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IP
• Clone: OTI1D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PLK1 Antibody (OTI1D4) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PLK1 Antibody (OTI1D4) - Azide and BSA Free (NBP1-48291) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PLK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:2000
• Theoretical MW: 68.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PLK1 Antibody (OTI1D4) - Azide and BSA Free

• cell cycle regulated protein kinase
• EC 2.7.11
• EC 2.7.11.21
• PLK
• PLK1
• PLK-1
• polo (Drosophia)-like kinase
• polo like kinase
• polo-like kinase 1polo-like kinase (Drosophila)
• Serine/threonine-protein kinase 13
• serine/threonine-protein kinase PLK1
• STPK13

Background

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PLK1