![Western Blot: PLEKHA7 Antibody [NBP3-12994] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PLEKHA7 antibody (NBP3-12994) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLEKHA7-Antibody-Western-Blot-NBP3-12994-img0003.jpg "Western Blot: PLEKHA7 Antibody [NBP3-12994] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with PLEKHA7 antibody (NBP3-12994) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Centrifuge briefly prior to opening. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PLEKHA7. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLEKHA7 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 127 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 20% Glycerol |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_15885.jpg)
![Immunohistochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunohistochemistry_15886.jpg)
![Immunocytochemistry E-Cadherin Antibody [Unconjugated]](https://images.novusbio.com/images2/ECadherin_AF748_Immunocytochemistry_16572.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PLEKHA7 |
![Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP3-12994] - PLEKHA7 antibody detects PLEKHA7 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded mouse colon. Green: PLEKHA7 stained by PLEKHA7 antibody (NBP3-12994) diluted at 1:1000. Red: E-Cadherin, stained byE-Cadherin antibody [GT477] (NBP2-42837) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PLEKHA7-Antibody-Immunohistochemistry-Paraffin-NBP3-12994-img0002.jpg "Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP3-12994] - PLEKHA7 antibody detects PLEKHA7 protein expression by immunohistochemical analysis. Sample: Paraffin-Embedded mouse colon. Green: PLEKHA7 stained by PLEKHA7 antibody (NBP3-12994) diluted at 1:1000. Red: E-Cadherin, stained byE-Cadherin antibody [GT477] (NBP2-42837) diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: PLEKHA7 Antibody [NBP3-12994] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLEKHA7 antibody (NBP3-12994) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PLEKHA7-Antibody-Western-Blot-NBP3-12994-img0001.jpg "Western Blot: PLEKHA7 Antibody [NBP3-12994] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PLEKHA7 antibody (NBP3-12994) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")