PMCA1 Antibody (5F10)

Images

 
Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin ...read more
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin ...read more
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin ...read more
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.
Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, ICC, IF
Clone
5F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PMCA1 Antibody (5F10) Summary

Immunogen
Purified human erythrocyte calcium ATPase.
Epitope
This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
Specificity
This detects all four known isoforms of the PMCA ATPase.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP2B1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Flow Cytometry 1 ug
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry 1:10 - 1:500
  • Immunofluorescence 1:100 - 1:200
Application Notes
Blocking/Neutralizing and ELISA usages were reported in scientific literature.
Publications
Read Publications using NB300-578.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 11022033). Porcine reactivity reported in scientific literature (PMID: 17680983). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites diluted with PBS
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PMCA1 Antibody (5F10)

  • ATPase, Ca++ transporting, plasma membrane 1
  • EC 3.6.3
  • EC 3.6.3.8
  • Plasma membrane calcium ATPase isoform 1
  • plasma membrane calcium ATPase
  • Plasma membrane calcium pump isoform 1
  • plasma membrane calcium pump
  • plasma membrane calcium-ATPase
  • PMCA1kb
  • PMCA1plasma membrane calcium-transporting ATPase 1

Background

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PMCA1 Antibody (NB300-578)(7)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PMCA1 Antibody (NB300-578) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ATP2B1
Entrez
Uniprot