PMCA1 Antibody (5F10) Summary
Purified human erythrocyte calcium ATPase.
This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
This detects all four known isoforms of the PMCA ATPase.
Test in a species/application not listed above to receive a full credit towards a future purchase.
Western Blot 1:1000 - 1:5000 Flow Cytometry 1 ug Immunocytochemistry/Immunofluorescence 1:10-1:500 Immunohistochemistry Immunohistochemistry-Frozen 1:10 - 1:500 Immunohistochemistry-Paraffin 1:500 Immunoprecipitation 1:10 - 1:500
Blocking/Neutralizing and ELISA usages were reported in scientific literature.
Publications using NB300-578 in the following applications:
Hamster reactivity reported in scientific literature (PMID: 11022033). Porcine reactivity reported in scientific literature (PMID: 17680983). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Alternate Names for PMCA1 Antibody (5F10)
ATPase, Ca++ transporting, plasma membrane 1 EC 3.6.3 EC 126.96.36.199 Plasma membrane calcium ATPase isoform 1 plasma membrane calcium ATPase Plasma membrane calcium pump isoform 1 plasma membrane calcium pump plasma membrane calcium-ATPase PMCA1kb PMCA1plasma membrane calcium-transporting ATPase 1 Background
The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
for 1 year from date of receipt.
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Publications for PMCA1 Antibody (NB300-578)(8)
We have publications tested in 1 confirmed species: Mouse.
We have publications tested in 1 application: WB.
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Publications using NB300-578
Ibeh CL, Yiu AJ, Kanaras YL et al. Evidence for a regulated Ca2+ entry in proximal tubular cells and its implication in calcium stone formation J. Cell. Sci. Mar 25 2019 [PMID: 30910829] (WB, Mouse) WB
Louch WE, Hougen K, Mork HK et al. Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout. J Physiol. 2010 Feb. [PMID: 20008467]
Sinkins WG, Estacion M, Prasad V et al. Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel. Am J Physiol Cell Physiol. 2009 Dec. [PMID: 19794142]
Hill JK, Williams DE, LeMasurier M et al. Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles. J Neurosci. 2006 Jun. [PMID: 16763025]
Krizaj D, Liu X, Copenhagen DR et al. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol. 2004 Aug. [PMID: 15236230]
Filoteo AG, Elwess NL, Enyedi A et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997 Sep. [PMID: 9295318]
Caride AJ, Filoteo AG, Enyedi A et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 1996 May [PMID: 8645230]
Borke JL, Caride A, Verma AK et al. Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules. Am J Physiol. 1989 Nov. [PMID: 2556040]
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