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PMCA1 Antibody (5F10)

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Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).
Western Blot: PMCA1 Antibody (5F10) [NB300-578] - Analysis of 25 ug of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3).
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin ...read more
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue).
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin ...read more
Immunocytochemistry/ Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin ...read more
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human tonsil tissues.
Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.
Flow Cytometry: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clone
5F10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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PMCA1 Antibody (5F10) Summary

Immunogen
Purified human erythrocyte calcium ATPase.
Epitope
This antibody recognizes an epitope between amino acids 724-783 of the human erythrocyte calcium pump.
Specificity
This detects all four known isoforms of the PMCA ATPase.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ATP2B1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:1000 - 1:5000
Application Notes
Blocking/Neutralizing and ELISA usages were reported in scientific literature.
Publications
Read Publications using
NB300-578 in the following applications:

  • WB
    1 publication

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 11022033). Porcine reactivity reported in scientific literature (PMID: 17680983). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PMCA1 Antibody (5F10)

  • ATPase, Ca++ transporting, plasma membrane 1
  • EC 3.6.3
  • EC 3.6.3.8
  • Plasma membrane calcium ATPase isoform 1
  • plasma membrane calcium ATPase
  • Plasma membrane calcium pump isoform 1
  • plasma membrane calcium pump
  • plasma membrane calcium-ATPase
  • PMCA1kb
  • PMCA1plasma membrane calcium-transporting ATPase 1

Background

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: PMCA2 Antibody [NB120-3529] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.
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Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image. Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.
Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.
Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.
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Immunocytochemistry/Immunofluorescence: PMCA3 Antibody [NBP1-87259] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human cerebellum shows strong positivity in neuronal processes.
Immunohistochemistry-Paraffin: PMCA3 Antibody [NBP1-87259] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
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Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Upregulation of miRNA inhibits the expression level of target genes in HK-2 cells and CKD patients. Western Blot shows that hsa-miR-4709-3p inhibit the protein level of ITGB8 and CALM3 respectively. NC: scrambled negative control transfection;4709: hsa-miR-4709-3p transfection. Image collected and cropped by CiteAb from the following publication (https://bmcnephrol.biomedcentral.com/articles/10.1186/s12882-019-1512-x) licensed under a CC-BY licence.
Western Blot: Calmodulin 3 Antibody [NBP2-15669] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.
(1 Publication)
Species: Hu, Mu, Rt
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Immunocytochemistry/Immunofluorescence: Calmodulin 2 Antibody [NBP2-14871] - DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 stained by Calmodulin 2 antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: Calmodulin 2 Antibody [NBP2-14871] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Calmodulin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
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Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.A2058 melanoma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. C6 glioma cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: Calmodulin Antibody (2D1) [NB120-2860] - Calmodulin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. HeLa Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Calmodulin at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
(2 Publications)
Species: Ba, Bv, Ch, Hu, Mu, Rt
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Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.
Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.
Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat brain lysate (left), 0.5 ug of His-tagged recombinant proteins (right) were probed with NBP2-50028 at 1:5,000. Lane 1: Parvalbumin, Lane 2: Calretinin, Lane 3: Calbindin.
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Immunohistochemistry-Paraffin: Sodium Calcium Exchanger 1/NCX1 Antibody [NBP2-94316] - Immunohistochemistry of paraffin-embedded mouse spleen using Sodium Calcium Exchanger 1/NCX1 Rabbit pAb (NBP2-94316) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - HeLa cells. Primary antibody at 1:100, secondary antibody: goat-anti mouse IgG AlexaFluor 568 at 1:100. ICC/IF image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: SERCA2 ATPase Antibody (2A7-A1) [NB300-581] - SERCA2 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA2 ATPase at a dilution of 1:100-1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
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Species: Am, Ca, Gp, Hu, Mu, Po, Rb, Rt, Sh
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Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.        
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12.5% SDS-PAGE Stained with Coomassie Blue.
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Immunohistochemistry: TLX2 Antibody [NBP2-82023] - Immunohistochemistry of TLX2 in rat brain tissue with TLX2 antibody at 2.5 ug/ml.
Immunocytochemistry/Immunofluorescence: TLX2 Antibody [NBP2-82023] - Immunofluorescence of TLX2 in rat brain tissue with TLX2 antibody at 20 ug/ml.
Species: Hu, Mu, Rt
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Flow Cytometry: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of Hela cells with Vitamin D Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse skin tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: VDR/NR1I1/Vitamin D Receptor Antibody (JA11-16) [NBP2-66778] - Analysis of paraffin-embedded mouse colon tissue using anti-Vitamin D Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Species: Hu, Mu, Rt
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Western Blot: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - ATP2A3 monoclonal antibody (M01), clone 2H3 Analysis of ATP2A3 expression in HL-60.
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Immunohistochemistry-Paraffin: SERCA3 ATPase Antibody (2H3) [H00000489-M01] - Analysis of monoclonal antibody to ATP2A3 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
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Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane, cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: nNOS Antibody [NB100-858] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: nNOS Antibody [NB100-858] - Wholemount preparations of the myenteric neurons in the distal colon segments following 3 (2a-2d) and 7 (4a-4d) days of repeated in vivo oxaliplatin administration compared to sham-treated animals at 3 (1a-1d) and 7 (3a-3d) days. Myenteric ganglia and neurons labeled with beta-Tubulin Tuj1 (red), nuclei labeled with DAPI (blue) (arrows) can be seen within the ganglion (1b). Smooth muscle cells (asterisks) are also labeled with DAPI (1b). Significant increase in the proportion of nNOS neurons (green) can be seen at both Days 3 and 7 after oxaliplatin treatment. Scale bar = 20 um. Image collected and cropped by CiteAb from the following publication (www.journal.frontiersin.org/article/10.3389/fnins.2013.00030/abstract) licensed under a CC-BY licence.
(1 Review)
(13 Publications)
Species: Gp, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC-WhMt, IHC, IHC-Fr, IHC-P, PEP-ELISA, WB
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Antibody (VE121G9)
NB300-622
Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Immunocytochemistry/Immunofluorescence: SERCA1 ATPase Antibody (VE121G9) [NB300-622] - SERCA1 ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing SERCA1 ATPase at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Species: Am, Ca, Hu, Mu, Rb, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
PMCA1 Antibody
(5F10)
NB300-578
PMCA1 Antibody (5F10)
Species: Hu, Mu, Rt, Po, Am, Ca, Ch, Fe, Ha, Pm, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP

Publications for PMCA1 Antibody (NB300-578)(8)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Showing Publications 1 - 8 of 8.
Publications using NB300-578 Applications Species
Ibeh CL, Yiu AJ, Kanaras YL et al. Evidence for a regulated Ca2+ entry in proximal tubular cells and its implication in calcium stone formation J. Cell. Sci. Mar 25 2019 [PMID: 30910829] (WB, Mouse) WB Mouse
Louch WE, Hougen K, Mork HK et al. Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout. J Physiol. 2010 Feb. [PMID: 20008467]
Sinkins WG, Estacion M, Prasad V et al. Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel. Am J Physiol Cell Physiol. 2009 Dec. [PMID: 19794142]
Hill JK, Williams DE, LeMasurier M et al. Splice-site A choice targets plasma-membrane Ca2+-ATPase isoform 2 to hair bundles. J Neurosci. 2006 Jun. [PMID: 16763025]
Krizaj D, Liu X, Copenhagen DR et al. Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum). J Comp Neurol. 2004 Aug. [PMID: 15236230]
Filoteo AG, Elwess NL, Enyedi A et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem. 1997 Sep. [PMID: 9295318]
Caride AJ, Filoteo AG, Enyedi A et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 1996 May [PMID: 8645230]
Borke JL, Caride A, Verma AK et al. Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules. Am J Physiol. 1989 Nov. [PMID: 2556040]

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Bioinformatics

Gene Symbol ATP2B1
Entrez
Uniprot