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CACNB2 Antibody (6C4)

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 (2 Reviews)
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Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Primary antibody dilution-1:1000 in blocking buffer, 5% Milk in TBS-0.05% Tween for 2 hours at RT. Blocking Overnightat +4 degreeC 2 Hours at RT. Secondary antibody: 1:2000 in blocking buffer, 1 hourat RT. Image from verified customer review.
Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Primary antibody dilution-1:1000 in blocking buffer, 5% Milk in TBS-0.05% Tween for 2 hours at RT. Blocking Overnightat +4 degreeC 2 Hours at RT. Secondary ...read more
Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - analysis of CACNB2 in HEK293 and CHO whole cell lysates using anti-CACNB2 antibody. Image from verified customer review.
Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in NIH/3T3 (Cat # L018V1).
Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in Hela S3 NE (Cat # L013V3).
ELISA: CACNB2 Antibody (6C4) [H00000783-M05] - Detection limit for recombinant GST tagged CACNB2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA
Clone
6C4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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CACNB2 Antibody (6C4) Summary

Immunogen
CACNB2 (NP_963890 213 a.a. - 301 a.a.) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PSSRKSTPPSSAIDIDATGLDAEENDIPANHRSPKPSANSVTSPHSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQ
Specificity
CACNB2 - calcium channel, voltage-dependent, beta 2 subunit
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CACNB2
Purity
IgG purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.
Reviewed Applications
Read 2 Reviews rated 4.5
using
H00000783-M05 in the following applications:
Publications
Read Publications using
H00000783-M05 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CACNB2 Antibody (6C4)

  • CAB2
  • CACNLB2
  • Calcium channel voltage-dependent subunit beta 2
  • calcium channel, voltage-dependent, beta 2 subunit
  • CAVB2
  • FLJ23743
  • Lambert-Eaton myasthenic syndrome antigen B
  • myasthenic (Lambert-Eaton) syndrome antigen B
  • MYSB
  • voltage-dependent L-type calcium channel subunit beta-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: TRNT1 Antibody [NBP1-86589] - Staining of human cell line A-431 shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRNT1 Antibody [NBP1-86589] - Staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
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Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP2-13954] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human skeletal muscle shows low expression as expected.
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Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: Nav1.5 Antibody (4G8-1G7) [NB110-61010] - Figure A: Plasma membrane localisation of Nav1.5 identified by 4G8:1G7 detected by anti-mouse Alexa Fluor-488 conjugated antibody; Figure B, DAP1 co-staining of cell nuclei
Flow Cytometry: Nav1.5 Antibody (4G8:1G7) [NB110-61010] - An intracellular stain was performed on U2OS cells with Nav1.5 Antibody [4G8:1G7] NB110-61010PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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Western Blot: CACNB4 Antibody (S10-7) [NBP2-12908] - analysis of hamster T-CHO cell lysate showing detection of CACNB4 Calcium Channel protein using Mouse Anti-CACNB4 Calcium Channel Monoclonal Antibody, Clone S10-7. Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-CACNB4 Calcium Channel Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
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Immunohistochemistry-Paraffin: CACNA1A Antibody [NBP2-38049] - Staining of human cerebral cortex shows high expression.
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Immunohistochemistry-Paraffin: CACNB1 Antibody [NBP2-76915] - Analysis of mouse brain tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CACNB1 Antibody [NBP2-76915] - Analysis of rat heart tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
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Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
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Publications for CACNB2 Antibody (H00000783-M05)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Reviews for CACNB2 Antibody (H00000783-M05) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using H00000783-M05:
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(2)
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(2)
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Images Ratings Applications Species Date Details
Western Blot CACNB2 H00000783-M05
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reviewed by:
Anmol Kumar
 WB Human 06/06/2018
View

Summary

ApplicationWestern Blot
Sample TestedARPE19 Cell lysate
SpeciesHuman
Lot07075-6C4
Western Blot CACNB2 H00000783-M05
Enlarge
reviewed by:
Abby Sewell
 WB Human 12/01/2014
View

Summary

ApplicationWestern Blot
Sample TestedHEK293 and CHO Whole cell lysates, CHO stably transfected with CACNA1C, CACNB2, and CACNA2D (ChanTest, CT-6004)
SpeciesHuman

Blocking

Blocking Details5% Non-fat Dry Milk in TBST, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:500, 2 hours at room temperature, 5% Non-fat dry milk in TBST

Secondary Antibody

Secondary DescriptionGoat anti-mouse IgG-HRP
Secondary Manufacturer Cat#sc-2005
Secondary Concentration1:10,000

Details

Detection NotesECL was used to visualize bands. Blots were imaged using a ChemiDoc XRS+ (Bio-Rad), exposure was 10 minutes.

Product General Protocols

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FAQs for CACNB2 Antibody (H00000783-M05) (0)

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Secondary Antibodies

 

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Western Blot CACNB2 H00000783-M05
Enlarge
 Anmol Kumar
06/06/2018
Application: WB
Species: Human
read this review
Western Blot CACNB2 H00000783-M05
Enlarge
 Abby Sewell
12/01/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol CACNB2
Entrez
OMIM
Uniprot
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