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ZNF652 Antibody

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Western Blot: ZNF652 Antibody [NBP1-97753] - 0.01ug/ml staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: ZNF652 Antibody [NBP1-97753] - 0.01ug/ml staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ZNF652 Antibody [NBP1-97753] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Po, BvSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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ZNF652 Antibody Summary

Immunogen
Peptide with sequence C-RHLAEKNSSAQHH corresponding to C-Terminus according to NP_055712.1.
Specificity
Reported variants represent identical protein: NP_055712.1, NP_001138837.1
Predicted Species
Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZNF652
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in human heart, kidney, and liver lysates is reported in scientific literature (PMID: 1696643).
Publications
Read Publications using
NBP1-97753 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ZNF652 Antibody

  • DKFZp781E2122
  • zinc finger protein 652

Background

ZNF652 functions as a transcriptional repressor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: CBFA2T3 Antibody [NBP2-57636] - Staining of human tonsil shows strong nuclear positivity in germinal and non-germinal center cells.
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Immunocytochemistry/Immunofluorescence: RUNX1T1/ETO Antibody [NBP2-55747] - Staining of human cell line HEK 293 shows localization to nucleoplasm. Antibody staining is shown in green.
Western Blot: RUNX1T1/ETO Antibody [NBP2-55747] - H2135 and H2087 non-small cell lung cancer cells showing baseline Runx1T1 expression versus Runx1T1 stable overexpression. Image submitted by a verified customer review.
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Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.
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Flow Cytometry: LNK Antibody (2D8) [NBP2-00641] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LNK antibody, and then analyzed by flow cytometry.
Western Blot: LNK Antibody (2D8) [NBP2-00641] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LNK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LNK.
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[p Ser213, p Ser210] ...
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Immunohistochemistry-Paraffin: MTHFR Antibody (5D3) [NBP2-37607] - Analysis of intima cancer tissues (left) and prostate tissues (right) using MTHFR mouse mAb with DAB staining.
ELISA: MTHFR Antibody (5D3) [NBP2-37607] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
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Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HeLa cells.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Ovary tissue.
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Western Blot: Radixin Antibody (1D9) [H00005962-M06] - Analysis of RDX expression in HeLa (Cat # L013V1).
Immunohistochemistry: Radixin Antibody (1D9) [H00005962-M06] - Decreased phosphorylation of radixin in the RMS after DX52-1 treatment. Merged image of radixin and phosphorylated rzrin/radixin/moesin (pERM) immunoreactivity in a coronal section of the control RMS. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fncel.2013.00161/abstract) licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: Radixin Antibody (1D9) [H00005962-M06] - Analysis of monoclonal antibody to RDX on HeLa cell. Antibody concentration 30 ug/ml
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Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
(4 Publications)
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Applications: ICC/IF, IHC, IHC-P, PLA, WB

Publications for ZNF652 Antibody (NBP1-97753)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol ZNF652
Entrez
Uniprot