![Immunocytochemistry/Immunofluorescence: PLEKHA7 Antibody [NBP2-76328] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-PLEKHA7 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/PLEKHA7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-76328-img0002.jpg "Immunocytochemistry/Immunofluorescence: PLEKHA7 Antibody [NBP2-76328] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-PLEKHA7 Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Partial recombinant human PLEKHA7 protein (amino acids 821-1121) [UniProt Q6IQ23} |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLEKHA7 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of Pig trabecular meshwork cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/images2/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.](http://images.novusbio.com/images2/Tight-Junction-Protein-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85047-img0008.jpg)
![Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85047] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/images2/Tight-Junction-Protein-1-Antibody-Immunohistochemistry-Paraffin-NBP1-85047-img0014.jpg)
![Knockout Validated: CSK Antibody [NBP2-92944] - Analysis of extracts from normal (control) and CSK knockout (KO) 293T cells, using CSK at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.De](http://images.novusbio.com/images2/CSK-Antibody-Knockout-Validated-NBP2-92944-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP2-92944] - Immunofluorescence analysis of C6 cells using [KO Validated] CSK Rabbit pAb (NBP2-92944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/CSK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92944-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CSK Antibody [NBP2-92944] - Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] CSK Rabbit pAb (NBP2-92944) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/CSK-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92944-img0003.jpg)
![Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.](http://images.novusbio.com/images2/HSD17B4-Antibody-(OTI4C4)-Immunofluorescence-NBP2-46005-img0001.jpg)
![Immunofluorescence: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of HeLa cells.](http://images.novusbio.com/images2/HSD17B4-Antibody-(OTI4C4)-Immunofluorescence-NBP2-46005-img0002.jpg)
![Immunohistochemistry: HSD17B4 Antibody (OTI4C4) [NBP2-46005] - Analysis of Human Ovary tissue.](http://images.novusbio.com/images2/HSD17B4-Antibody-(OTI4C4)-Immunohistochemistry-NBP2-46005-img0003.jpg)
![Immunohistochemistry: D Box Binding Protein Antibody [NBP2-83997] - Human Liver](http://images.novusbio.com/images2/D-Box-Binding-Protein-Antibody-Immunohistochemistry-NBP2-83997-img0001.jpg)
![Western Blot: D Box Binding Protein Antibody [NBP2-83997] - WB Suggested Anti-DBP Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate](http://images.novusbio.com/images2/D-Box-Binding-Protein-Antibody-Western-Blot-NBP2-83997-img0002.jpg)
![Western Blot: D Box Binding Protein Antibody [NBP2-83997] - Host: Rabbit. Target Name: DBP. Sample Tissue: Human Hela Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/images2/D-Box-Binding-Protein-Antibody-Western-Blot-NBP2-83997-img0004.jpg)
![Immunohistochemistry-Paraffin: PMCA1 Antibody (5F10) [NB300-578] - Both normal and cancer biopsies of deparaffinized Human heart tissues.](http://images.novusbio.com/images2/PMCA1-Antibody-(5F10)-Immunohistochemistry-Paraffin-NB300-578-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/PMCA1-Antibody-(5F10)-Immunocytochemistry-Immunofluorescence-NB300-578-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMCA1 Antibody (5F10) [NB300-578] - Analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/images2/PMCA1-Antibody-(5F10)-Immunocytochemistry-Immunofluorescence-NB300-578-img0005.jpg)
![Western Blot: CGNL1 Antibody [NBP2-37959] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG](http://images.novusbio.com/images2/CGNL1-Antibody-Western-Blot-NBP2-37959-img0003.jpg)
![Immunohistochemistry-Paraffin: CGNL1 Antibody [NBP2-37959] - Analysis in human kidney and skeletal muscle tissues. Corresponding CGNL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/CGNL1-Antibody-Immunohistochemistry-Paraffin-NBP2-37959-img0008.jpg)
![Immunohistochemistry-Paraffin: CGNL1 Antibody [NBP2-37959] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/images2/CGNL1-Antibody-Immunohistochemistry-Paraffin-NBP2-37959-img0009.jpg)
![Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/p120-catenin-Antibody-(2E8)-Immunohistochemistry-NBP2-45646-img0001.jpg)
![Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/p120-catenin-Antibody-(2E8)-Immunohistochemistry-NBP2-45646-img0002.jpg)
![Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/p120-catenin-Antibody-(2E8)-Immunohistochemistry-NBP2-45646-img0003.jpg)
![Flow Cytometry: LNK Antibody (2D8) [NBP2-00641] - Analysis of Hela cells, using anti-LNK antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/images/LNK-Antibody-(2D8)-Flow-Cytometry-NBP2-00641-img0001.jpg)
![Flow Cytometry: LNK Antibody (2D8) [NBP2-00641] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LNK antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/images/LNK-Antibody-(2D8)-Flow-Cytometry-NBP2-00641-img0002.jpg)
![Western Blot: LNK Antibody (2D8) [NBP2-00641] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LNK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LNK.](http://images.novusbio.com/images/LNK-Antibody-(2D8)-Western-Blot-NBP2-00641-img0003.jpg)
![Western Blot: Rac1 Antibody [NB100-91266] - WB on tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution: 1:1000 incubated overnight at 4C.](http://images.novusbio.com/images2/Rac1-Antibody-Western-Blot-NB100-91266-img0011.jpg)
![Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat spinal cord (ventral horn) using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/images2/Rac1-Antibody-Immunohistochemistry-NB100-91266-img0005.jpg)
![Immunohistochemistry: Rac1 Antibody [NB100-91266] - Rat ligated sciatic nerve using Rabbit antibody to RAC1, RAC2 whole serum at 1:1000 dilution. Pre-absorption of the antibody with the immunizing peptide completely abolishes the immunostaining (not shown).](http://images.novusbio.com/images2/Rac1-Antibody-Immunohistochemistry-NB100-91266-img0004.jpg)
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Sample (30 ug of whole cell lysate) A: A431 B: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/Catenin-alpha-1-Antibody-Western-Blot-NBP2-15723-img0001.jpg)
![Western Blot: Catenin alpha 1 Antibody [NBP2-15723] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/Catenin-alpha-1-Antibody-Western-Blot-NBP2-15723-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Catenin alpha 1 Antibody [NBP2-15723] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/Catenin-alpha-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15723-img0004.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for PLEKHA7 Antibody (NBP2-76328)Discover more about diseases related to PLEKHA7 Antibody (NBP2-76328).
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| Gene Symbol | PLEKHA7 |
![Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP2-76328] - IHC analysis of a formalin fixed paraffin embedded tissue section of human stomach using PLEKHA7 (NBP2-76328) antibody at 1:200 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin.](http://images.novusbio.com/fullsize2/PLEKHA7-Antibody-Immunohistochemistry-Paraffin-NBP2-76328-img0001.jpg "Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP2-76328] - IHC analysis of a formalin fixed paraffin embedded tissue section of human stomach using PLEKHA7 (NBP2-76328) antibody at 1:200 dilution. The staining was developed using HRP-DAB detection method and the nuclei were counterstained with hematoxylin.")
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in 13 year old testis. Immunoexpression in Leydig cell. Image from a verified customer review.](http://images.novusbio.com/images2/Cytochrome-P450-17A1-Antibody-(OTI3F11)-Immunohistochemistry-NBP2-01151-img0025.jpg)
![Immunohistochemistry: Cytochrome P450 17A1 Antibody (OTI3F11) [NBP2-01151] - Staining in macaque Leydig cells. Image from a verified customer review.](http://images.novusbio.com/images2/Cytochrome-P450-17A1-Antibody-(OTI3F11)-Immunohistochemistry-NBP2-01151-img0026.jpg)
![Flow Cytometry: Cytochrome P450 17A1 Antibody (3F11) [NBP2-01151] - Analysis of Hela cells, using anti-Cytochrome P450 17A1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/images2/Cytochrome-P450-17A1-Antibody-(3F11)-Flow-Cytometry-NBP2-01151-img0013.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Primary antibody dilution-1:1000 in blocking buffer, 5% Milk in TBS-0.05% Tween for 2 hours at RT. Blocking Overnightat +4 degreeC 2 Hours at RT. Secondary antibody: 1:2000 in blocking buffer, 1 hourat RT. Image from verified customer review.](http://images.novusbio.com/images2/CACNB2-Antibody-(6C4)-Western-Blot-H00000783-M05-img0007.jpg)
![Western Blot: CACNB2 Antibody (6C4) [H00000783-M05] - Analysis of CACNB2 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/images2/CACNB2-Antibody-(6C4)-Western-Blot-H00000783-M05-img0006.jpg)
![ELISA: CACNB2 Antibody (6C4) [H00000783-M05] - Detection limit for recombinant GST tagged CACNB2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/images2/CACNB2-Antibody-(6C4)-ELISA-H00000783-M05-img0003.jpg)
![Western Blot: Vitamin D BP Antibody [NBP1-88027] - Analysis using Anti-GC antibody NBP1-88027 (A) shows similar pattern to independent antibody NBP1-88028 (B).](http://images.novusbio.com/images2/Vitamin-D-BP-Antibody-Western-Blot-NBP1-88027-img0008.jpg)
![Western Blot: Vitamin D BP Antibody [NBP1-88027] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/images2/Vitamin-D-BP-Antibody-Western-Blot-NBP1-88027-img0007.jpg)
![Western Blot: Vitamin D BP Antibody [NBP1-88027] - Equine adult and embryonic liver cell lysates. WB image submitted by a verified customer review.](http://images.novusbio.com/images2/Vitamin-D-BP-Antibody-Western-Blot-NBP1-88027-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/images2/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0008.jpg)
![Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.](http://images.novusbio.com/images2/RhoA-Antibody-Immunohistochemistry-Paraffin-NB100-91273-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.](http://images.novusbio.com/images2/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NB100-91273-img0022.jpg)
![Western Blot: KIFC3 Antibody [NBP2-17058] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/images2/KIFC3-Antibody-Western-Blot-NBP2-17058-img0001.jpg)
![Immunohistochemistry-Paraffin: KIFC3 Antibody [NBP2-17058] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/images2/KIFC3-Antibody-Immunohistochemistry-Paraffin-NBP2-17058-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: KIFC3 Antibody [NBP2-17058] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/images2/KIFC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17058-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ULK4 Antibody [NBP1-20229] - At E12.5, Ulk4 (green) was expressed in Ki67 positive cells (red). This image was submitted via customer Review.](http://images.novusbio.com/images2/ULK4-Antibody-Immunocytochemistry-NBP1-20229-img0001.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/images2/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89600-img0009.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining of human small intestine shows high expression.](http://images.novusbio.com/images2/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89600-img0010.jpg)
![Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/Cingulin-Antibody-Immunohistochemistry-Paraffin-NBP1-89600-img0011.jpg)
![Western Blot: ZNF652 Antibody [NBP1-97753] - 0.01ug/ml staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/images/ZNF652-Antibody-Western-Blot-NBP1-97753-img0001.jpg)
![Immunohistochemistry-Paraffin: ZNF652 Antibody [NBP1-97753] - Staining of Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/images2/ZNF652-Antibody-Immunohistochemistry-Paraffin-NBP1-97753-img0002.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](http://images.novusbio.com/images2/Goat-anti-Rabbit-IgG-(H+L)-Secondary-Antibody-[HRP]-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](http://images.novusbio.com/images2/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")