Plastin L Antibody - Azide and BSA Free

Western Blot: Plastin L Antibody [NBP2-99366] - Anti-Plastin L rabbit polyclonal antibody at 1:500 dilution. Lane A: THP1 Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: Daudi Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 70 kDa

Immunoprecipitation: Plastin L Antibody [NBP2-99366] - Plastin L was immunoprecipitated using: Lane A: 0.5 mg mouse lung tissue Lysate 4 ul anti-Plastin L rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Plastin L rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 30 kDa

Immunocytochemistry/Immunofluorescence: Plastin L Antibody [NBP2-99366] - Immunofluorescence staining of Plastin L in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99366 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Plastin L Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Plastin L.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LCP1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunoprecipitation 5-10 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Plastin L Antibody - Azide and BSA Free

• CP64
• DKFZp781A23186
• FLJ25423
• FLJ26114
• FLJ39956
• LC64PPLS2bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin))
• LCP-1
• LPL
• L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P)
• L-PLASTIN
• lymphocyte cytosolic protein 1 (L-plastin)
• Lymphocyte cytosolic protein 1
• Lymphocyte cytosolic protein-1 (plasmin)
• plastin 2
• plastin-2

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCP1