PKA C alpha Antibody - Azide and BSA Free

Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PKA C alpha Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human PKA C alpha.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKACA
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PKA C alpha Antibody - Azide and BSA Free

• cAMP-dependent protein kinase catalytic subunit alpha
• Cs
• EC 2.7.11
• EC 2.7.11.11
• MGC102831
• MGC48865
• PKA C alpha
• PKA C-alpha
• Pkaca
• PKCA1
• PKCD
• PRKACA
• protein kinase A catalytic subunit
• protein kinase, cAMP-dependent, catalytic, alpha

Background

PKA (Protein Kinase A), also known as cAMP-dependent protein kinase, phosphorylates serine or threonine residues in target protein in response to elevated levels of cAMP. Inactive PKA exists as a tetrameric protein composed of two regulatory (R) subunits and two catalytic (C) subunits. Activation occurs when two cAMP molecules bind to each R subunit which causes a conformational change that releases the active C subunits.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKACA