PKA 2 beta Antibody - BSA Free

Western Blot: PKA 2 beta Antibody [NBP3-12636] - Anti-PKA 2 beta rabbit polyclonal antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP3-12636] - Immunochemical staining of human PKA 2 beta in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Western Blot: PKA 2 beta Antibody [NBP3-12636] - Lane A: NIH/3T3 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:54 kDa

Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP3-12636] - Staining of human PRKAR2B in human adrenal gland with rabbit polyclonal antibody at 1:1000.

Immunoprecipitation: PKA 2 beta Antibody [NBP3-12636] - Lane A:0.5 mg NIH/3T3 Whole Cell Lysate4 uL anti-PRKAR2B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PRKAR2B rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :54 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PKA 2 beta Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human PKA 2 beta fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKAR2B
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for PKA 2 beta Antibody - BSA Free

• cAMP-dependent protein kinase type II-beta regulatory chain
• cAMP-dependent protein kinase type II-beta regulatory subunit
• H_RG363E19.2
• PKA 2 beta
• PRKAR2
• PRKAR2B
• protein kinase, cAMP-dependent, regulatory, type II, beta
• RII-BETA
• WUGSC:H_RG363E19.2

Background

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAR2B