PKA RI beta Antibody [Unconjugated]

Western blot shows lysates of MDA-MB-468 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, SW480 human colorectal adenocarcinoma cell line, Raji human Burkitt's lymphoma cell line, and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows recombinant human PKA RIa, PKA RI beta , and PKA RII beta (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Human/ Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PKA RI beta was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for PKA RI beta at approximately 56 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat PKA RI beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4177) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

PKA RI beta Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human PKA RI beta
  Met1-Val381
  Accession # P31321
• Specificity: Detects human, mouse, and rat PKA RI beta in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA RI alpha or rhPKA RII beta is observed; cross-reactivity with PKA RII alpha is unknown.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PRKAR1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PKA RI beta Antibody [Unconjugated]

• PKA RI beta
• PRKAR1
• PRKAR1B
• RIbeta

Background

The cAMP-dependent protein kinase (PKA) holoenzyme is composed of two regulatory and two catalytic subunits, designated PKA R and PKA C, respectively. PKA R subunits exist as two classes, RI and RII, with each class containing two isoforms, alpha  and beta . Upon PKA R subunit binding to the second messenger cAMP, active PKA C subunits are released, initiating a phosphorylation cascade that regulates such cellular functions as metabolism, ion transport, and gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PKA RI beta Antibody (AF4177)(3)

Publications using AF4177

• X Wang, VM Philip, G Ananda, CC White, A Malhotra, PJ Michalski, KRM Karuturi, SR Chintalapu, C Acklin, M Sasner, DA Bennett, PL De Jager, GR Howell, GW Carter A Bayesian Framework for Generalized Linear Mixed Modeling Identifies New Candidate Loci for Late-Onset Alzheimer's Disease Genetics, 2018-03-05;0(0):. 2018-03-05 [PMID: 29507048] (IHC-Fr, Mouse)
• Ronit Ilouz, Varda Lev-Ram, Eric A Bushong, Travis L Stiles, Dinorah Friedmann-Morvinski, Christopher Douglas et al. Isoform-specific subcellular localization and function of protein kinase A identified by mosaic imaging of mouse brain eLife 2017-01-12 [PMID: 28079521]
• Gao Feng microRNA-208a in an early stage myocardial infarction rat model and the effect on cAMP-PKA signaling pathway Mol Med Rep, 2016-06-14;0(0):. 2016-06-14 [PMID: 27314868] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PRKAR1B
• Uniprot:
  • P31321()