PITX2 Antibody [Alexa Fluor® 488]

• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

PITX2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human PITX2
  Ala149-Val317
  Accession # Q99697
• Specificity: Detects human PITX2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human PITX1 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PITX2 (PITuitary homeoboX 2; also ARP1 and RIEG) is a 33-36 kDa nuclear member of the bicoid subfamily, paired homeobox family of transcription factors. It is widely expressed in the embryo, and serves as the earliest known marker for pituitary gland development. A long isoform commonly called PITX2C also plays a very key role in the establishment of left-right organ asymmetry. PITX2 appears in multiple germ layer derivatives, and it appears that one of its important functions is to integrating retinoic acid and Wnt/ beta -catenin signaling. The N- and C-terminal regions of PITX2 normally interact noncovalently, rendering PITX2 inactive.  Over aa 149‑317, human and mouse PITX2 are identical in aa sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.