COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: H7147
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Suitable Sample Type: 23015

COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated] Summary

• Immunogen: Recombinant human COUP-TF II/NR2F2
  Amino Acids 43-64
• Specificity: This antibody specifically recognizes human COUP-TF II and cross-reacts with rat COUP-TF II. This antibody does not cross-react with human COUP-TF I or EAR2. Not yet tested in other species.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR2F2
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
• Buffer: A liquid formulation in physiologic saline with 0.1% NaN₃.
• Preservative: 0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]

• Apolipoprotein A-I regulatory protein 1
• ARP-1
• ARP1ADP-ribosylation factor related protein 1
• chicken ovalbumin upstream promoter transcription factor 2
• COUP transcription factor 2
• COUP transcription factor II
• COUPTF II
• COUP-TF II
• COUP-TF2
• COUPTFB
• COUPTFII
• COUP-TFII
• MGC117452
• NR2F2
• Nuclear receptor subfamily 2 group F member 2
• nuclear receptor subfamily 2, group F, member 2
• SVP40
• TFCOUP2apolipoprotein AI regulatory protein 1

Background

Chicken ovalbumin upstream promoter transcription factor II (COUP-TF II, ARP-1, COUP-TFB; NR2F2) is a member of the orphan nuclear receptorfamily. COUP-TF II is expressed in the tongue, follicles of vibrissae, cochIea and in stroma of the nasal septum. COUP-TF II has roles in angiogenesis, vascular remodeling and heart development. COUP-TFs were shown to interact with a number of other nuclear receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for COUP-TF II/NR2F2 Antibody (PP-H7147-00)(29)

Publications using PP-H7147-00

• J Li, A Wiesinger, L Fokkert, BJ Boukens, AO Verkerk, VM Christoffe, GJJ Boink, HD Devalla Molecular and electrophysiological evaluation of human cardiomyocyte subtypes to facilitate generation of composite cardiac models Journal of tissue engineering, 2022-10-18;13(0):2041731422112. 2022-10-18 [PMID: 36277058] (ICC, Human)
• D Yang, J Gomez-Garc, S Funakoshi, T Tran, I Fernandes, GD Bader, MA Laflamme, GM Keller Modeling human multi-lineage heart field development with pluripotent stem cells Cell Stem Cell, 2022-09-01;29(9):1382-1401.e8. 2022-09-01 [PMID: 36055193] (ICC, Human)
• KF Rodriguez, PR Brown, CM Amato, B Nicol, CF Liu, X Xu, HH Yao Somatic cell fate maintenance in mouse fetal testes via autocrine/paracrine action of AMH and activin B Nature Communications, 2022-07-15;13(1):4130. 2022-07-15 [PMID: 35840551] (IHC, Mouse)
• X Zhu, Y Guo, C Chu, D Liu, K Duan, Y Yin, C Si, Y Kang, J Yao, X Du, J Li, S Zhao, Z Ai, Q Zhu, W Ji, Y Niu, T Li BRN2 as a key gene drives the early primate telencephalon development Science Advances, 2022-03-04;8(9):eabl7263. 2022-03-04 [PMID: 35245119] (IHC, Primate (Macaca fascicularis))
• K Sasaki, A Oguchi, K Cheng, Y Murakawa, I Okamoto, H Ohta, Y Yabuta, C Iwatani, H Tsuchiya, T Yamamoto, Y Seita, M Saitou The embryonic ontogeny of the gonadal somatic cells in mice and monkeys Cell Reports, 2021-05-04;35(5):109075. 2021-05-04 [PMID: 33951437] (IHC, Cynomolgus Monkey)
• L Wang, CM Cheng, J Qin, M Xu, CY Kao, J Shi, E You, W Gong, LP Rosa, P Chase, L Scampavia, F Madoux, T Spicer, P Hodder, HE Xu, SY Tsai, MJ Tsai Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment Sci Adv, 2020-04-29;6(18):eaaz8031. 2020-04-29 [PMID: 32494682] (ChIP, Human)
• L Wang, CM Cheng, J Qin, M Xu, CY Kao, J Shi, E You, W Gong, LP Rosa, P Chase, L Scampavia, F Madoux, T Spicer, P Hodder, HE Xu, SY Tsai, MJ Tsai Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment Sci Adv, 2020;6(18):eaaz8031. 2020 [PMID: 32494682] (ChIP, Human)
• SF Sorrells, MF Paredes, D Velmeshev, V Herranz-Pé, K Sandoval, S Mayer, EF Chang, R Insausti, AR Kriegstein, JL Rubenstein, J Manuel Gar, EJ Huang, A Alvarez-Bu Immature excitatory neurons develop during adolescence in the human amygdala Nat Commun, 2019-06-21;10(1):2748. 2019-06-21 [PMID: 31227709] (IHC, Human)
• S Bonney, BJC Dennison, M Wendlandt, JA Siegenthal Retinoic Acid Regulates Endothelial ?-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature Front Cell Neurosci, 2018-12-05;12(0):476. 2018-12-05 [PMID: 30568578] (IHC, Human)
• M Absinta, SK Ha, G Nair, P Sati, NJ Luciano, M Palisoc, A Louveau, KA Zaghloul, S Pittaluga, J Kipnis, DS Reich Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI Elife, 2017-10-03;6(0):. 2017-10-03 [PMID: 28971799] (IHC-P, Human, Primate)
• JJ Hwa, N Beckouche, L Huang, Y Kram, H Lindskog, RA Wang Abnormal arterial-venous fusions and fate specification in mouse embryos lacking blood flow Sci Rep, 2017-09-20;7(1):11965. 2017-09-20 [PMID: 28931948] (IHC, Mouse)
• JH Lee, SI Protze, Z Laksman, PH Backx, GM Keller Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations Cell Stem Cell, 2017-08-03;21(2):179-194.e4. 2017-08-03 [PMID: 28777944] (ICC, Human)
• R Smith, YT Huang, T Tian, D Vojtasova, O Mesalles-N, SM Pollard, T Pratt, DJ Price, V Fotaki The transcription factor Foxg1 promotes optic fissure closure in the mouse by suppressing Wnt8b in the nasal optic stalk J. Neurosci., 2017-07-20;0(0):. 2017-07-20 [PMID: 28729440] (IHC, Mouse)
• Ayman Alzu'bi Distinct cortical and sub-cortical neurogenic domains for GABAergic interneuron precursor transcription factors NKX2.1, OLIG2 and COUP-TFII in early fetal human telencephalon Brain Struct Funct, 2016-11-30;0(0):. 2016-11-30 [PMID: 27905023] (Human)
• COUP-TFII regulates satellite cell function and muscular dystrophy J Clin Invest, 2016-09-12;0(0):. 2016-09-12 [PMID: 27617862] (ChIP, IHC, Mouse)
• Diverse Functions of Retinoic Acid in Brain Vascular Development J Neurosci, 2016-07-20;36(29):7786-801. 2016-07-20 [PMID: 27445154] (IHC-Fr, Mouse)
• SC Lin, CY Kao, HJ Lee, CJ Creighton, MM Ittmann, SJ Tsai, SY Tsai, MJ Tsai Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer Nat Commun, 2016-04-25;7(0):11418. 2016-04-25 [PMID: 27108958] (Western Blot, Human)
• Tanwar V, Bylund J, Hu J, Yan J, Walthall J, Mukherjee A, Heaton W, Wang W, Potet F, Rai M, Kupershmidt S, Knapik E, Hatzopoulos A Gremlin 2 promotes differentiation of embryonic stem cells to atrial fate by activation of the JNK signaling pathway. Stem Cells, 2014-07-01;32(7):1774-88. 2014-07-01 [PMID: 24648383] (IHC, Mouse)
• Lindskog , Henrik, Kim , Yung Hae, Jelin , Eric B, Kong , Yupeng, Guevara-Gallardo , Salvador, Kim , Tyson N, Wang , Rong A Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals. Development, 2014-03-01;141(5):1120-8. 2014-03-01 [PMID: 24550118] (IHC, Mouse)
• Boyer-Di Ponio J, El-Ayoubi F, Glacial F, Ganeshamoorthy K, Driancourt C, Godet M, Perriere N, Guillevic O, Couraud P, Uzan G Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS ONE, 2014-01-02;9(1):e84179. 2014-01-02 [PMID: 24392113] (ICC, Mouse)
• Litchfield L, Appana S, Datta S, Klinge C COUP-TFII inhibits NFkappaB activation in endocrine-resistant breast cancer cells. Mol Cell Endocrinol, 2013-10-17;382(1):358-67. 2013-10-17 [PMID: 24141032] (Immunoprecipitation, Western Blot, Human)
• Mohammed H Endogenous Purification Reveals GREB1 as a Key Estrogen Receptor Regulatory Factor. Oncogene, 2013-02-09;3(2):342-9. 2013-02-09 [PMID: 23403292] (ChIP, Human)
• Litchfield LM, Riggs KA, Hockenberry AM, Oliver LD, Barnhart KG, Cai J, Pierce WM, Ivanova MM, Bates PJ, Appana SN, Datta S, Kulesza P, McBryan J, Young LS, Klinge CM Identification and characterization of nucleolin as a COUP-TFII coactivator of retinoic acid receptor β transcription in breast cancer cells. PLoS ONE, 2012-05-31;7(5):e38278. 2012-05-31 [PMID: 22693611] (ICC, Human)
• Mayer S, Roeser M, Lachmann P Chicken Ovalbumin Upstream Promoter Transcription Factor II Regulates Renin Gene Expression. J. Biol. Chem., 2012-05-29;287(29):24483-91. 2012-05-29 [PMID: 22645148] (Western Blot, Human, Mouse)
• van den Driesche S, Walker M, McKinnell C, Scott HM, Eddie SL, Mitchell RT, Seckl JR, Drake AJ, Smith LB, Anderson RA, Sharpe RM Proposed role for COUP-TFII in regulating fetal Leydig cell steroidogenesis, perturbation of which leads to masculinization disorders in rodents. PLoS ONE, 2012-05-17;7(5):e37064. 2012-05-17 [PMID: 22615892] (IHC, Human, Rat)
• Rosa A, Brivanlou AH A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. EMBO J., 2010-12-10;30(2):237-48. 2010-12-10 [PMID: 21151097] (Western Blot, Human)
• Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 2010-04-01;120(5):1694-707. 2010-04-01 [PMID: 20364082] (IHC-P, Western Blot, Mouse)
• Aerbajinai W, Zhu J, Kumkhaek C, Chin K, Rodgers GP SCF induces gamma-globin gene expression by regulating downstream transcription factor COUP-TFII. Blood, 2009-04-28;114(1):187-94. 2009-04-28 [PMID: 19401563] (Western Blot, Human)
• Perilhou A, Tourrel-Cuzin C, Kharroubi I, Henique C, Fauveau V, Kitamura T, Magnan C, Postic C, Prip-Buus C, Vasseur-Cognet M The transcription factor COUP-TFII is negatively regulated by insulin and glucose via Foxo1- and ChREBP-controlled pathways. Mol. Cell. Biol., 2008-09-02;28(21):6568-79. 2008-09-02 [PMID: 18765640] (Western Blot, Mouse)

Reviews for COUP-TF II/NR2F2 Antibody (PP-H7147-00) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Mouse, Rat.

reviewed by: Bhavin Shah IHC-Fr Mouse 05/13/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: E17 coronal brain section
• Species: Mouse
• Lot: A-2

Comments

• Comments: Brain sections were antigen retrieved with 10mM sodium citrate (pH6) buffer and then blocked with 10% donkey serum in PBST. primary and secondary antibodies were prepared in the same blocking buffer.

reviewed by: cynthia morimoto IHC-P Rat 02/25/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 22253897
• Species: Rat

reviewed by: cynthia morimoto IF Rat 02/25/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22615892
• Species: Rat

Bioinformatics

• Gene Symbol: NR2F2