COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clone
H7147
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Suitable Sample Type
23015

Order Details

COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated] Summary

Immunogen
Recombinant human COUP-TF II/NR2F2
Amino Acids 43-64
Specificity
This antibody specifically recognizes human COUP-TF II and cross-reacts with rat COUP-TF II. This antibody does not cross-react with human COUP-TF I or EAR2. Not yet tested in other species.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NR2F2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
  • Immunoprecipitation
Reviewed Applications
Read 3 Reviews rated 4.3
using
PP-H7147-00 in the following applications:

Publications
Read Publications using
PP-H7147-00 in the following applications:

Packaging, Storage & Formulations

Storage
This antibody is stable for greater than six months when held at -20° C in a manual defrost freezer or at -70° C. Upon thawing, the antibody can be stored at 2° - 8° C for at least 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Buffer
A liquid formulation in physiologic saline with 0.1% NaN3.
Preservative
0.1% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for COUP-TF II/NR2F2 Antibody (H7147) [Unconjugated]

  • Apolipoprotein A-I regulatory protein 1
  • ARP-1
  • ARP1ADP-ribosylation factor related protein 1
  • chicken ovalbumin upstream promoter transcription factor 2
  • COUP transcription factor 2
  • COUP transcription factor II
  • COUPTF II
  • COUP-TF II
  • COUP-TF2
  • COUPTFB
  • COUPTFII
  • COUP-TFII
  • MGC117452
  • NR2F2
  • Nuclear receptor subfamily 2 group F member 2
  • nuclear receptor subfamily 2, group F, member 2
  • SVP40
  • TFCOUP2apolipoprotein AI regulatory protein 1

Background

Chicken ovalbumin upstream promoter transcription factor II (COUP-TF II, ARP-1, COUP-TFB; NR2F2) is a member of the orphan nuclear receptorfamily. COUP-TF II is expressed in the tongue, follicles of vibrissae, cochIea and in stroma of the nasal septum. COUP-TF II has roles in angiogenesis, vascular remodeling and heart development. COUP-TFs were shown to interact with a number of other nuclear receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for COUP-TF II/NR2F2 Antibody (PP-H7147-00)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ChIP, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
ChIP
(2)
ICC
(3)
IHC
(6)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(6)
All Applications
Filter By Species
Human
(12)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using PP-H7147-00 Applications Species
S Bonney, BJC Dennison, M Wendlandt, JA Siegenthal Retinoic Acid Regulates Endothelial ?-catenin Expression and Pericyte Numbers in the Developing Brain Vasculature Front Cell Neurosci, 2018;12(0):476. 2018 [PMID: 30568578] (IHC, Human) IHC Human
M Absinta, SK Ha, G Nair, P Sati, NJ Luciano, M Palisoc, A Louveau, KA Zaghloul, S Pittaluga, J Kipnis, DS Reich Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI Elife, 2017;6(0):. 2017 [PMID: 28971799] (IHC-P, Human) IHC-P Human
JJ Hwa, N Beckouche, L Huang, Y Kram, H Lindskog, RA Wang Abnormal arterial-venous fusions and fate specification in mouse embryos lacking blood flow Sci Rep, 2017;7(1):11965. 2017 [PMID: 28931948] (IHC, Mouse) IHC Mouse
JH Lee, SI Protze, Z Laksman, PH Backx, GM Keller Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations Cell Stem Cell, 2017;21(2):179-194.e4. 2017 [PMID: 28777944] (ICC, Human) ICC Human
R Smith, YT Huang, T Tian, D Vojtasova, O Mesalles-N, SM Pollard, T Pratt, DJ Price, V Fotaki The transcription factor Foxg1 promotes optic fissure closure in the mouse by suppressing Wnt8b in the nasal optic stalk J. Neurosci., 2017;0(0):. 2017 [PMID: 28729440] (IHC, Mouse) IHC Mouse
Ayman Alzu'bi Distinct cortical and sub-cortical neurogenic domains for GABAergic interneuron precursor transcription factors NKX2.1, OLIG2 and COUP-TFII in early fetal human telencephalon Brain Struct Funct, 2016;0(0):. 2016 [PMID: 27905023] (Human) Human
COUP-TFII regulates satellite cell function and muscular dystrophy J Clin Invest, 2016;0(0):. 2016 [PMID: 27617862] (ChIP, Mouse) ChIP Mouse
Diverse Functions of Retinoic Acid in Brain Vascular Development J Neurosci, 2016;36(29):7786-801. 2016 [PMID: 27445154] (IHC-Fr, Mouse) IHC-Fr Mouse
SC Lin, CY Kao, HJ Lee, CJ Creighton, MM Ittmann, SJ Tsai, SY Tsai, MJ Tsai Dysregulation of miRNAs-COUP-TFII-FOXM1-CENPF axis contributes to the metastasis of prostate cancer Nat Commun, 2016;7(0):11418. 2016 [PMID: 27108958] (Western Blot, Human) Western Blot Human
Tanwar V, Bylund J, Hu J, Yan J, Walthall J, Mukherjee A, Heaton W, Wang W, Potet F, Rai M, Kupershmidt S, Knapik E, Hatzopoulos A Gremlin 2 promotes differentiation of embryonic stem cells to atrial fate by activation of the JNK signaling pathway. Stem Cells, 2014;32(7):1774-88. 2014 [PMID: 24648383] (IHC, Mouse) IHC Mouse
Show All 21 Publications.

Reviews for COUP-TF II/NR2F2 Antibody (PP-H7147-00) (3) 4.33

Average Rating: 4.3
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Mouse, Rat.

Reviews using PP-H7147-00:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen COUP-TF II/NR2F2 PP-H7147-00
Enlarge
reviewed by:
Anonymous
IHC-Fr Mouse 05/13/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedE17 coronal brain section
SpeciesMouse
LotA-2

Comments

CommentsBrain sections were antigen retrieved with 10mM sodium citrate (pH6) buffer and then blocked with 10% donkey serum in PBST. primary and secondary antibodies were prepared in the same blocking buffer.
 
reviewed by:
Anonymous
IHC-P Rat 02/25/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22253897
SpeciesRat
Special ApplicationsIndependent review from antibodyresource.comPublished in PMID 22253897.
 
reviewed by:
Anonymous
IF Rat 02/25/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22615892
SpeciesRat

Blocking

Blocking DetailsSee PMID 22615892

Details

Detection NotesSee PMID 22615892

Comments

CommentsIndependent review from antibodyresource.comPublished in PMID 22615892

Product General Protocols

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Video Protocols

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FAQs for COUP-TF II/NR2F2 Antibody (PP-H7147-00) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for COUP-TF II/NR2F2 Antibody (PP-H7147-00)

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Recent Reviews

4.3
5
1
4
2
3
0
2
0
1
0

Anonymous
05/13/2019
Application: IHC-Fr
Species: Mouse

 
Anonymous
02/25/2015
Application: IHC-P
Species: Rat

 
Anonymous
02/25/2015
Application: IF
Species: Rat

Bioinformatics

Gene Symbol NR2F2