FoxC1 Antibody


Western Blot: FoxC1 Antibody [NB100-1268] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 Antibody (1 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag more
Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 more
Flow Cytometry: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary more

Product Details

Reactivity Hu, Mu, ZeSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC-Fr, IHC-P, PEP-ELISA
0.5 mg/ml

Order Details

FoxC1 Antibody Summary

Peptide with sequence RTSGAFVYDCSKF corresponding to C-Terminus according to NP_001444.2.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.5 - 1.5 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
ChIP: An anonymous customer has reported successful usage on human cell line. ChIP successful usage on
WB: In transfected HEK293 transiently expressing full-length human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa band was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. A customer has reported positive results on human breast cancer cells (T47D).Use in Immunohistochemistry-paraffin reported in multiple pieces of scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 23862012).
FoxC1 Lysate (NBL1-10801)
Read Publications using
NB100-1268 in the following applications:

Reactivity Notes

Zebrafish embryos is reported in scientific literature (PMID: 17000708). Mouse reactivity reported in multiple pieces of scientific literature. Expected from sequence similarity:Rat.

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for FoxC1 Antibody

  • ARA
  • FKHL7
  • forkhead box C1
  • forkhead box protein C1
  • forkhead, drosophila, homolog-like 7
  • forkhead/winged helix-like transcription factor 7
  • forkhead-related activator 3
  • Forkhead-related protein FKHL7
  • Forkhead-related transcription factor 3
  • FoxC1
  • FREAC3
  • FREAC-3
  • IGDA
  • IHG1
  • IRID1
  • myeloid factor-delta
  • RIEG3


This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Species: Hu, Mu, Rt, Av, Bv, Pm
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Species: Mu
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Species: Mu
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Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Species: Hu, Mu
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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IP
Species: Hu, Mu, Rt, Bv
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Species: Hu, Mu, Ze
Applications: WB, ChIP, ICC/IF, IHC-Fr, IHC-P, PEP-ELISA

Publications for FoxC1 Antibody (NB100-1268)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-Fr, IHC-P.

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Showing Publications 1 - 10 of 10.
Publications using NB100-1268 Applications Species
Hirukawa A, Smith HW, Zuo D et al. Targeting EZH2 reactivates a breast cancer subtype-specific anti-metastatic transcriptional program. Nat Commun. Jun 29 2018 [PMID: 29959321] (IHC-P, Mouse) IHC-P Mouse
Wang X, Shan X, Gregory-Evans CY. A mouse model of aniridia reveals the in vivo downstream targets of Pax6 driving iris and ciliary body development in the eye. Biochim. Biophys. Acta Oct 20 2016 [PMID: 27771509] (IHC-P) IHC-P
Wang J, Xiao Y, Hsu CW et al. Yap and Taz play a crucial role in neural crest-derived craniofacial development. Development. 2016 Feb 01 [PMID: 26718006] (IHC-P, Mouse) IHC-P Mouse
Siegenthaler JA, Choe Y, Patterson KP et al. Foxc1 is required by pericytes during fetal brain angiogenesis. Biol Open 2013 May 20 [PMID: 23862012] (IHC-Fr, Mouse) IHC-Fr Mouse
Hayashi H, Kume T. Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells. PLoS One 11;3(6):e2401. 2008 Jun. [PMID: 18545664]
Berry FB, Skarie JM, Mirzayans F, Fortin Y, Hudson TJ, Raymond V, Link BA, Walter MA. FOXC1 is required for cell viability and resistance to oxidative stress in the eye through the transcriptional regulation of FOXO1A. Hum Mol Genet15;17(4):490-505. 2008 Feb. [PMID: 17993506]
Zarbalis, K et al. Cortical dysplasia skull defects in mice with a Foxc1 allele reveal the role of meningeal differentiation in regulating cortical development. PNAS, Aug; 104: 14002 - 14007. 2007 [PMID: 17715063]
Tamimi Y, Skarie JM, Footz T, Berry FB, Link BA, Walter MA. FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. um Mol Genet 1;15(21):3229-40. 2006 Nov. [PMID: 17000708]
Berry FB, Lines MA, Oas JM, Footz T, Underhill DA, Gage PJ, Walter MA. Functional interactions between FOXC1 and PITX2 underlie the sensitivity to FOXC1 gene dose in Axenfeld-Rieger syndrome and anterior segment dysgenesis. Hum Mol Genet15;15(6):905-19. 2006 Mar. [PMID: 16449236]
Zhou Y, Kato H, Asanoma K et al. Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth. Genomics 2002 Nov [PMID: 12408963]

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FoxC1 Antibody (NB100-1268) (0)

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Control Lysate(s)

Secondary Antibodies


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Gene Symbol FOXC1