FoxC1 Antibody

Images

 
Western Blot: FoxC1 Antibody [NB100-1268] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 Antibody (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag ...read more
Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, ZeSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FoxC1 Antibody Summary

Immunogen
Peptide with sequence RTSGAFVYDCSKF corresponding to C-Terminus according to NP_001444.2.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.5 ug/ml
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 3-5 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
ChIP: An anonymous customer has reported successful usage on human cell line. ChIP successful usage on
WB: In transfected HEK293 transiently expressing full-length human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa band was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. An anonymous customer has reported positive results on human breast cancer cells (T47D).
IHC-P: Human kidney shows nuclear staining in DCT and select nuclear staining in the glomerulus. An anonymous customer has reported successful usage in mouse (periocular mesenchyme, cornea).
Positive Control
FoxC1 Lysate (NBL1-10801)
Publications
Read Publications using
NB100-1268 in the following applications:

Reactivity Notes

Zebrafish embryos is reported in scientific literature (PMID: 17000708). Expected from sequence similarity: Mouse

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxC1 Antibody

  • ARA
  • FKHL7
  • FKHL7RIEG3
  • forkhead box C1
  • forkhead box protein C1
  • forkhead, drosophila, homolog-like 7
  • forkhead/winged helix-like transcription factor 7
  • forkhead-related activator 3
  • Forkhead-related protein FKHL7
  • Forkhead-related transcription factor 3
  • FoxC1
  • FREAC3
  • FREAC-3
  • FREAC3IRID1
  • IGDA
  • IHG1
  • IRID1
  • myeloid factor-delta
  • RIEG3

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxC1 Antibody (NB100-1268)(9)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC-Fr, IHC-P.


Filter By Application
IHC-Fr
(1)
IHC-P
(2)
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Mouse
(2)
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Showing Publications 1 - 9 of 9.
Publications using NB100-1268 Applications Species
Wang X, Shan X, Gregory-Evans CY. A mouse model of aniridia reveals the in vivo downstream targets of Pax6 driving iris and ciliary body development in the eye. Biochim. Biophys. Acta Oct 20 2016 [PMID: 27771509] (IHC-P) IHC-P
Wang J, Xiao Y, Hsu CW et al. Yap and Taz play a crucial role in neural crest-derived craniofacial development. Development. 2016 Feb 01 [PMID: 26718006] (IHC-P, Mouse) IHC-P Mouse
Siegenthaler JA, Choe Y, Patterson KP et al. Foxc1 is required by pericytes during fetal brain angiogenesis. Biol Open 2013 May 20 [PMID: 23862012] (IHC-Fr, Mouse) IHC-Fr Mouse
Hayashi H, Kume T. Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells. PLoS One 11;3(6):e2401. 2008 Jun. [PMID: 18545664]
Berry FB, Skarie JM, Mirzayans F, Fortin Y, Hudson TJ, Raymond V, Link BA, Walter MA. FOXC1 is required for cell viability and resistance to oxidative stress in the eye through the transcriptional regulation of FOXO1A. Hum Mol Genet15;17(4):490-505. 2008 Feb. [PMID: 17993506]
Zarbalis, K et al. Cortical dysplasia skull defects in mice with a Foxc1 allele reveal the role of meningeal differentiation in regulating cortical development. PNAS, Aug; 104: 14002 - 14007. 2007 [PMID: 17715063]
Tamimi Y, Skarie JM, Footz T, Berry FB, Link BA, Walter MA. FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. um Mol Genet 1;15(21):3229-40. 2006 Nov. [PMID: 17000708]
Berry FB, Lines MA, Oas JM, Footz T, Underhill DA, Gage PJ, Walter MA. Functional interactions between FOXC1 and PITX2 underlie the sensitivity to FOXC1 gene dose in Axenfeld-Rieger syndrome and anterior segment dysgenesis. Hum Mol Genet15;15(6):905-19. 2006 Mar. [PMID: 16449236]
Zhou Y, Kato H, Asanoma K et al. Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth. Genomics 2002 Nov [PMID: 12408963]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FoxC1 Antibody (NB100-1268) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FOXC1
Entrez
Uniprot