FoxC1 Antibody

Images

 
Biological Strategies: Western Blot: FoxC1 Antibody [NB100-1268] - Left- Significant upregulation of Foxc1 mRNA in Ezh2-/- Tet-ON PyVmT endpoint tumours compared to wild-type tumours. Right- Immunoblot of Ezh2+/+ ...read more
Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 ...read more
Orthogonal Strategies: Immunohistochemistry: FoxC1 Antibody [NB100-1268] - Representative images of formalin fixed paraffin-embedded tissue samples from human Luminal B tumours stained for FOXC1 and EZH2. Scale ...read more
Flow Cytometry: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary ...read more
Genetic Strategies: Knockdown Validated: FoxC1 Antibody [NB100-1268] - Left: Representative immunoblot showing Foxc1 expression in cells stably transduced with a control shRNA (shLacZ) or two independent shRNAs ...read more

Product Details

Summary
Reactivity Hu, Mu, ZeSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, KD, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

FoxC1 Antibody Summary

Immunogen
FoxC1 Antibody is made to a peptide with sequence RTSGAFVYDCSKF corresponding to C-Terminus according to NP_001444.2.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
FOXC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1.5 ug/mL
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunocytochemistry/Immunofluorescence 10 ug/mL
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Knockdown Validated
  • Knockout Validated
Application Notes
FoxC1 Antibody validated for ChIP from a verified customer review.A customer has reported positive results on human breast cancer cells (T47D). Use in IHC-P reactivity reported in (:29959321). Use in IHC-Fr reported in scientific literature (PMID: 23862012).
Control
FoxC1 Overexpression Lysate
Publications
Read Publications using
NB100-1268 in the following applications:

Reactivity Notes

Zebrafish embryos reactivity is reported in scientific literature (PMID: 17000708). Mouse reactivity reported (PMID: 29959321). Expected reactivity from sequence similarity: Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FoxC1 Antibody

  • ARA
  • FKHL7
  • FKHL7RIEG3
  • forkhead box C1
  • forkhead box protein C1
  • forkhead, drosophila, homolog-like 7
  • forkhead/winged helix-like transcription factor 7
  • forkhead-related activator 3
  • Forkhead-related protein FKHL7
  • Forkhead-related transcription factor 3
  • FoxC1
  • FREAC3
  • FREAC-3
  • FREAC3IRID1
  • IGDA
  • IHG1
  • IRID1
  • myeloid factor-delta
  • RIEG3

Background

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxC1 Antibody (NB100-1268)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ChIP, ICC/IF, IHC-Fr, IHC-P, KO, WB.


Filter By Application
ChIP
(1)
ICC/IF
(1)
IHC-Fr
(2)
IHC-P
(3)
KO
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB100-1268 Applications Species
Lee YH, Lee HT, Chen CL et al. Role of FOXC1 in regulating APSCs self-renewal via STI-1/PrPC signaling. Theranostics. 2019 Aug 15 [PMID: 31588228] (ChIP, WB, ICC/IF, KO, Mouse) ChIP, WB, ICC/IF, KO Mouse
Hirukawa A, Smith HW, Zuo D et al. Targeting EZH2 reactivates a breast cancer subtype-specific anti-metastatic transcriptional program. Nat Commun. Jun 29 2018 [PMID: 29959321] (IHC-P, Mouse) IHC-P Mouse
Lin Yu-Jung, Shyu Woei-Cherng, Chang Chi-Wei et al. Tumor Hypoxia Regulates Forkhead Box C1 to Promote Lung Cancer Progression. Theranostics 2018 Mar 5 [PMID: 28435458] (WB, IHC-Fr, Human) WB, IHC-Fr Human
Wang X, Shan X, Gregory-Evans CY. A mouse model of aniridia reveals the in vivo downstream targets of Pax6 driving iris and ciliary body development in the eye. Biochim. Biophys. Acta Oct 20 2016 [PMID: 27771509] (IHC-P) IHC-P
Wang J, Xiao Y, Hsu CW et al. Yap and Taz play a crucial role in neural crest-derived craniofacial development. Development. 2016 Feb 01 [PMID: 26718006] (IHC-P, Mouse) IHC-P Mouse
Siegenthaler JA, Choe Y, Patterson KP et al. Foxc1 is required by pericytes during fetal brain angiogenesis. Biol Open 2013 May 20 [PMID: 23862012] (IHC-Fr, Mouse) IHC-Fr Mouse
Hayashi H, Kume T. Foxc transcription factors directly regulate Dll4 and Hey2 expression by interacting with the VEGF-Notch signaling pathways in endothelial cells. PLoS One 11;3(6):e2401. 2008 Jun. [PMID: 18545664]
Berry FB, Skarie JM, Mirzayans F, Fortin Y, Hudson TJ, Raymond V, Link BA, Walter MA. FOXC1 is required for cell viability and resistance to oxidative stress in the eye through the transcriptional regulation of FOXO1A. Hum Mol Genet15;17(4):490-505. 2008 Feb. [PMID: 17993506]
Zarbalis, K et al. Cortical dysplasia skull defects in mice with a Foxc1 allele reveal the role of meningeal differentiation in regulating cortical development. PNAS, Aug; 104: 14002 - 14007. 2007 [PMID: 17715063]
Tamimi Y, Skarie JM, Footz T, Berry FB, Link BA, Walter MA. FGF19 is a target for FOXC1 regulation in ciliary body-derived cells. um Mol Genet 1;15(21):3229-40. 2006 Nov. [PMID: 17000708]
Show All 12 Publications.

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for FoxC1 Antibody (NB100-1268) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol FOXC1
Entrez
Uniprot