Western Blot: FoxC1 Antibody [NB100-1268] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 Antibody (1 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag ...read more
Immunocytochemistry/ Immunofluorescence: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 ...read more
Flow Cytometry: FoxC1 Antibody [NB100-1268] - Paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary antibody at 10 ug/mL, 1 hr incubation, followed by Alexa Fluor 488 secondary ...read more
ChIP: An anonymous customer has reported successful usage on human cell line. ChIP successful usage on WB: In transfected HEK293 transiently expressing full-length human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa band was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. A customer has reported positive results on human breast cancer cells (T47D).Use in Immunohistochemistry-paraffin reported in multiple pieces of scientific literature. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 23862012).
Zebrafish embryos is reported in scientific literature (PMID: 17000708). Mouse reactivity reported in multiple pieces of scientific literature. Expected from sequence similarity:Rat.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris (pH 7.3), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for FoxC1 Antibody
forkhead box C1
forkhead box protein C1
forkhead, drosophila, homolog-like 7
forkhead/winged helix-like transcription factor 7
forkhead-related activator 3
Forkhead-related protein FKHL7
Forkhead-related transcription factor 3
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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