Western Blot: FoxC1 Antibody [NB100-1268] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 Antibody (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag ...read more
Immunohistochemistry-Paraffin: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: FoxC1 Antibody [NB100-1268] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody ...read more
Immunohistochemistry-Paraffin: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ChIP: An anonymous customer has reported successful usage on human cell line. ChIP successful usage on WB: In transfected HEK293 transiently expressing full-length human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa band was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. An anonymous customer has reported positive results on human breast cancer cells (T47D). IHC-P: Human kidney shows nuclear staining in DCT and select nuclear staining in the glomerulus. An anonymous customer has reported successful usage in mouse (periocular mesenchyme, cornea).
Zebrafish embryos is reported in scientific literature (PMID: 17000708). Expected from sequence similarity: Mouse, Rat.
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for FoxC1 Antibody
forkhead box C1
forkhead box protein C1
forkhead, drosophila, homolog-like 7
forkhead/winged helix-like transcription factor 7
forkhead-related activator 3
Forkhead-related protein FKHL7
Forkhead-related transcription factor 3
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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