Western Blot: FoxC1 Antibody [NB100-1268] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human FOXC1 with DYKDDDDK tag probed with FOXC1 Antibody (1ug/ml) in Lane A and probed with anti- DYKDDDDK Tag ...read more
Immunohistochemistry-Paraffin: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Kidney.
Western Blot: FoxC1 Antibody [NB100-1268] - Human Bone Marrow lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: FoxC1 Antibody [NB100-1268] - Staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining
ChIP: An anonymous customer has reported successful usage on human cell line. ChIP successful usage on Zebrafish embryos is reported in scientific literature (PMID: 17000708). WB: In transfected HEK293 transiently expressing full-length human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa band was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. An anonymous customer has reported positive results on human breast cancer cells (T47D). IHC-P: Human kidney shows nuclear staining in DCT and select nuclear staining in the glomerulus. An anonymous customer has reported successful usage in mouse (periocular mesenchyme, cornea).
Read Publications using NB100-1268 in the following applications:
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.