PINK1 Antibody (8E10.1D6)


Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of (A) Partial Recombinant Human PINK-1 protein with estimated molecular weight at 13kDa and (B) Human Liver lysate using PINK1 antibody clone 8E10.1D6 at more
Biological Strategies: Western Blot: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Whole cell protein from HeLa cells treated with or without valinomycin (1 uM, 24h) as indicated was separated by SDS-PAGE on a 7.5% more
Immunohistochemistry-Paraffin: PINK1 Antibody (8E10.1D6) [NBP2-36488] - Analysis of FFPE tissue section of human hepatocellular carcinoma using PINK1 antibody (clone 8E10.1D6) at 5 ug/ml concentration. The cancer cells more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: PINK1 Antibody (8E10.1D6) [NBP2-36488] - HeLa cells were treated with valinomycin (1 uM, 24h) prior to being fixed in 10% buffered formalin for 10 more

Product Details

Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PAGE
1.0 mg/ml
Validated by:

Biological Strategies


PINK1 Antibody (8E10.1D6) Summary

PINK1 antibody was developed using a synthetic peptide made to the human PINK1 protein sequence (between residues 100-250). [Swiss-Prot: Q9BXM7]
Mitochondrion outer membrane, Cytoplasm
Human PINK1 protein sequence (between residues 100-250), only reactive to isoform 1.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
IgG2b Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 2-4 ug/ml
  • Immunocytochemistry/Immunofluorescence 20-50 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • SDS-Page
Application Notes
Unprocessed PINK1 is 63 kDa which undergoes proteolytic processing to generate 55 kDa and 42 kDa cleaved forms, and bands at the mentioned positions may be expected in Western blot application. Use in SDS-Page reported in scientific literature (PMID: 27553674).
Theoretical MW
62.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
PINK1 Overexpression Lysate (Denatured)
PINK1 Overexpression Lysate
PINK1 Knockout 293T Cell Lysate
Read Publications using
NBP2-36488 in the following applications:

  • 1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29486776).

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for PINK1 Antibody (8E10.1D6)

  • BRPK
  • EC
  • FLJ27236
  • PARK6
  • Parkinson disease (autosomal recessive) 6
  • PINK1 monoclonal
  • PINK1
  • protein kinase BRPK
  • PTEN Induced Kinase 1
  • PTEN induced putative kinase 1
  • PTEN-induced putative kinase protein 1
  • serine/threonine-protein kinase PINK1, mitochondrial


Phosphatase and Tensin Homolog (PTEN) is a tumor suppressor which acts as an antagonist to phosphatidylinositol 3-kinase (PI3K) signaling. PTEN exerts enzymatic activity as a phosphatidylinositol-3,4,5-trisphosphate (PIP3) phosphatase, opposing PI3K activity by reducing availability of PIP3 to proliferating cells. Loss of PTEN function leads to elevated PIP3 and increased activation of PI3K/AKT signaling in many types of cancer.

PINK1 (PTEN induced putative kinase 1) protein contains a N-terminal mitochondrial targeting sequence, putative transmembrane helix, linker region, serine (Ser65)/threonine (Thr257) kinase domain and C-terminal segment. PINK1 is translated in the cytosol, then translocated to the outer mitochondrial membrane where it is rapidly cleaved and degraded as a part of normal mitochondrial function. In damaged (depolarized) mitochondria, PINK1 becomes stabilized and accumulates, resulting in the subsequent phosphorylation of numerous proteins on the mitochondrial surface.

When PINK1 is imported into the cell, mitochondrial processing peptidase, presenilin-associated rhomboid-like protease and AFG3L2 cleave PINK1 and tag it for the ubiquitin-proteasome pathway, keeping low PINK1 protein expression at basal conditions (1,2). Accumulation of PINK1 in mitochondria indicate damage. PINK1 maintains mitochondrial function/integrity, provides protection against mitochondrial dysfunction during cellular stress, and is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) (3). PINK1 has a theoretical molecular weight of 63 kDa and undergoes proteolytic processing to generate at least two cleaved forms (55 kDa and 42 kDa).

Ultimately PARK2 (E3 Ubiquitin Ligase Parkin) is recruited to the damaged mitochondria where it is activated by 1) PINK-mediated phosphorylation of PARK2 at serine 65, and 2) PARK2 interaction with phosphorylated ubiquitin (also phosphorylated by PINK1 on serine 65) (4,5). There is a strong interplay between Parkin and PINK1, where loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by Parkin (2,4,5). Mutations in either Parkin or PINK1 alter mitochondrial turnover, resulting in the accumulation of defective mitochondria and, ultimately, neurodegeneration in Parkinson's disease. Mutations in the PINK1 gene located within the PARK6 locus on chromosome 1p35-p36 have been identified in patients with early-onset Parkinson's disease (6).


1.Rasool, S., Soya, N., Truong, L., Croteau, N., Lukacs, G. L., & Trempe, J. F. (2018). PINK1 autophosphorylation is required for ubiquitin recognition. EMBO Rep, 19(4). doi:10.15252/embr.201744981

2.Shiba-Fukushima, K., Arano, T., Matsumoto, G., Inoshita, T., Yoshida, S., Ishihama, Y.,... Imai, Y. (2014). Phosphorylation of mitochondrial polyubiquitin by PINK1 promotes Parkin mitochondrial tethering. PLoS Genet, 10(12), e1004861. doi:10.1371/journal.pgen.1004861

3.Vives-Bauza, C., Zhou, C., Huang, Y., Cui, M., de Vries, R. L., Kim, J.,... Przedborski, S. (2010). PINK1-dependent recruitment of Parkin to mitochondria in mitophagy. Proc Natl Acad Sci U S A, 107(1), 378-383. doi:10.1073/pnas.0911187107

4.McWilliams, T. G., Barini, E., Pohjolan-Pirhonen, R., Brooks, S. P., Singh, F., Burel, S.,... Muqit, M. M. K. (2018). Phosphorylation of Parkin at serine 65 is essential for its activation in vivo. Open Biol, 8(11). doi:10.1098/rsob.180108

5.Exner, N., Treske, B., Paquet, D., Holmstrom, K., Schiesling, C., Gispert, S.,... Haass, C. (2007). Loss-of-function of human PINK1 results in mitochondrial pathology and can be rescued by parkin. J Neurosci, 27(45), 12413-12418. doi:10.1523/jneurosci.0719-07.2007

6.Valente, E. M., Bentivoglio, A. R., Dixon, P. H., Ferraris, A., Ialongo, T., Frontali, M.,... Wood, N. W. (2001). Localization of a novel locus for autosomal recessive early-onset parkinsonism, PARK6, on human chromosome 1p35-p36. Am J Hum Genet, 68(4), 895-900. doi:10.1086/319522


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PINK1 Antibody (NBP2-36488)(4)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PINK1 Antibody (NBP2-36488). (Showing 1 - of FAQs).

    Control Lysate(s)

    Secondary Antibodies


    Isotype Controls

    Other Available Formats

    Alexa Fluor 350 NBP2-36488AF350
    Alexa Fluor 405 NBP2-36488AF405
    Alexa Fluor 488 NBP2-36488AF488
    Alexa Fluor 532 NBP2-36488AF532
    Alexa Fluor 594 NBP2-36488AF594
    Alexa Fluor 647 NBP2-36488AF647
    Alexa Fluor 700 NBP2-36488AF700
    Alexa Fluor 750 NBP2-36488AF750
    Biotin NBP2-36488B
    DyLight 350 NBP2-36488UV
    DyLight 405 NBP2-36488V
    DyLight 488 NBP2-36488G
    DyLight 550 NBP2-36488R
    DyLight 594 NBP2-36488DL594
    DyLight 650 NBP2-36488C
    DyLight 680 NBP2-36488FR
    DyLight 755 NBP2-36488IR
    FITC NBP2-36488F
    HRP NBP2-36488H
    Janelia Fluor 549 NBP2-36488JF549
    Janelia Fluor 646 NBP2-36488JF646

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    Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
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    There's an autophagy for that!
    By Christina Towers, PhDA critical mechanism that cells use to generate nutrients and fuel metabolism is through a process called autophagy.  This process is complex and involves over 20 different proteins, most of which are highly conserved acro...  Read full blog post.

    The role of Parkin and autophagy in retinal pigment epithelial cell (RPE) degradation
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    The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
    Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.

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    PINK1 and its role in Parkinson's disease
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    Gene Symbol PINK1