Phosphoserine phosphatase Antibody

Images

 
Western Blot: Phosphoserine phosphatase Antibody [NBP1-56848] - Analysis of PSPH in human PSPH overexpressed glioma (Lane1) and non-transfected (Lane2) cells using anti-PSPH (Phosphoserine phosphatase) antibody. Image ...read more
Western Blot: Phosphoserine phosphatase Antibody [NBP1-56848] - Human Heart lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Phosphoserine phosphatase Antibody Summary

Immunogen
Synthetic peptides corresponding to PSPH(phosphoserine phosphatase) The peptide sequence was selected from the middle region of PSPH. Peptide sequence IMIGDGATDMEACPPADAFIGFGGNVIRQQVKDNAKWYITDFVELLGELE.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSPH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against PSPH and was validated on Western blot.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-56848 in the following application:

Publications
Read Publication using
NBP1-56848 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Phosphoserine phosphatase Antibody

  • L-3-phosphoserine phosphatase
  • O-phosphoserine phosphohydrolase
  • phosphoserine phosphatase
  • PSPase
  • PSPEC 3.1.3.3

Background

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved i

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

Publications for Phosphoserine phosphatase Antibody (NBP1-56848)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
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Review for Phosphoserine phosphatase Antibody (NBP1-56848) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-56848:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Phosphoserine phosphatase NBP1-56848
Enlarge
reviewed by:
Anonymous
WB Human 06/04/2016
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Summary

ApplicationWestern Blot
Sample TestedHuman glioma cells transfected with PSPH ORF (Lane1) or non trasfected (Lane2)
SpeciesHuman

Blocking

Blocking Details5% non fat dried milk in TBS-T

Primary Anitbody

Dilution Ratio1:500 in 3% BSA in TBS, 4 degree overnight incubation

Secondary Antibody

Secondary DescriptionGoat anti rabbit-HRP
Secondary Manufacturer Cat#31460
Secondary Concentration1:10,000

Details

Detection NotesBlot was incubated with ECL-femto (Pierce) for 2 min. X-ray film was overlaid in dark and processed through Kodak x-omat.

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FAQs for Phosphoserine phosphatase Antibody (NBP1-56848) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
06/04/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol PSPH
Uniprot