Recombinant Human ASIP/Agouti Protein, CF

Images

 
Recombinant Human ASIP/Agouti (Catalog # 9094-AG) inhibits alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human ASIP/Agouti Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human ASIP protein
His23-Cys132
Accession #
N-terminal Sequence
His23
Protein/Peptide Type
Recombinant Proteins
Gene
ASIP
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-23 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ASIP/Agouti Protein, CF

  • agouti (mouse)-signaling protein
  • agouti signaling protein
  • agouti signaling protein, nonagouti homolog (mouse)
  • agouti signaling protein, nonagouti homolog
  • Agouti switch protein
  • agouti-signaling protein
  • AGSW
  • AGTI
  • AGTIL
  • AGTILMGC126092
  • ASIP
  • ASPAGSW
  • MGC126093
  • nonagouti homolog
  • SHEP9

Background

Agouti, also known as Agouti Signaling Protein (ASIP), is a 132 amino peptide that regulates hair pigmentation in mice and adipocyte metabolism in humans (1). Mature human Agouti shares 78% and 76% amino acid sequence identity with mouse and rat Agouti, respectively (2, 3). Human Agouti is primarily expressed in adipose tissue, whereas its adipose expression in mice is only induced by mutations in the Agouti promoter (4, 5). Agouti is also expressed in the pancreas where it enhances the production of insulin by beta cells (6). Agouti functions as a competitive antagonist at several melanocortin receptors. It blocks ACTH binding to MC1R and MSH binding to MC1R, MC3R, and MC4R (7-9). It blocks MSH induced effects on melanocytes including proliferation and melanin production (3, 7, 8).
  1. Voisey, J. and A. van Daal (2002) Pigment Cell Res. 15:10.
  2. Kwon, H.Y. et al. (1994) Proc. Natl. Acad. Sci. USA 91:9760.
  3. Wilson, B.D. et al. (1995) Hum. Mol. Genet. 4:223.
  4. Mynatt, R.L. et al. (1997) Proc. Natl. Acad. Sci. USA 94:919.
  5. Mynatt, R.L. and J.M. Stephens (2001) Am. J. Physiol. Cell Physiol. 280:C954.
  6. Xue, B.Z. et al. (1999) Physiol. Genomics 1:11.
  7. Lu, D. et al. (1994) Nature 371:799.
  8. Suzuki, I. et al. (1997) J. Invest. Dermatol. 108:838.
  9. Kiefer, L.L. et al. (1997) Biochemistry 36:2084.

Customers Who Viewed This Item Also Viewed...

NB100-1533
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NLS1040
Species: Hu, Pm
Applications: IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-540
Species: Mu
Applications: Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-67232
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88370
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-41103
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, IF
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
NBP1-89133
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-1519
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, PEP-ELISA, IHC-FrFl
9094-AG
Species: Hu
Applications: Bioactivity

Publications for ASIP (9094-AG) (0)

There are no publications for ASIP (9094-AG).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ASIP (9094-AG) (0)

There are no reviews for ASIP (9094-AG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for ASIP (9094-AG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human ASIP/Agouti Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol ASIP
Uniprot