ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [Unconjugated]

Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line, Saos-2 human osteosarcoma cell line, and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for a-2,8-Sialyltransferase 8B/ST8SIA2 at approximately 63 to 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

a-2,8-Sialyltransferase 8B/ ST8SIA2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human a-2,8-Sialyltransferase 8B/ ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

IMR‑32 human neuroblastoma cell line was stained with Sheep Anti-Human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6590, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, IHC, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human alpha -2,8‑Sialyltransferase 8B/ST8SIA2
  Asp24-Thr375
  Accession # Q92186
• Specificity: Detects human alpha -2,8‑Sialyltransferase 8B/ST8SIA2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ST8SIA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry 5-15 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody [Unconjugated]

• alpha-2,8-sialyltransferase 8B 1
• alpha-2,8-sialyltransferase 8B
• EC 2.4.99
• EC 2.4.99.-
• HsT19690
• MGC116854
• MGC116857
• sialyltransferase 8 (alpha-2, 8-sialytransferase) B
• Sialyltransferase 8B
• Sialyltransferase X
• Sialytransferase St8Sia II
• SIAT8B
• SIAT8-B
• ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 2
• ST8SIA2
• ST8SiaII
• ST8SIA-II
• STX
• STXST8SiaII

Background

ST8SIA2, also known as sialyltransferase X, is mainly expressed during embryonic development (4) and shows strict preference on NCAM (6). Polysialic acid (PSA), abundant on the neural cell adhesion molecule (NCAM) during embryonic development, acts as an anti-adhesive glycan to negatively modulate the adhesive properties of NCAM (1). PSA expression decreases promptly after birth, and becomes restricted to the hippocampus, hypothalamus, and olfactory bulb, areas of the brain that require continuous cell migration and synaptic plasticity (2). Expression of PSA in cancer cells has been suggested to increase tumor invasiveness and to promote tumor growth (3). The temporal regulation of PSA is dependent on the expression of two polysialyltransferases, ST8SIA4 and ST8SIA2 (4, 5). The high degree of substrate specificity is achieved through specific enzyme-substrate recognition at both the protein sequence and glycan structure levels (6, 7). Like most glycosyltransferases, ST8SIA2 is a Golgi‑resident type II membrane protein. The activity of this enzyme has been measured with a phosphatase-coupled method (8).

1. Scheidegger, E.P. et al. (1995) J. Biol. Chem. 270:22685.
2. Rutishauser, U. (2008) Nat. Rev. Neurosci. 9:26.
3. Seidenfaden, R. et al. (2003) Mol.Cell. Biol. 23, 5908.
4. Angata, K. et al. (1997) J. Biol. Chem. 272:7182.
5. Ong, E. et al. (1998). Glycobiology 8:415.
6. Korima, N. et al. (1996) J. Biol. Chem. 271:19457.
7. Thompson, M. G. et al. (2010) J. Biol. Chem. in press.
8. Wu, Z.L. et al. (2010) Glycobiology doi: 10.1093/glycob/cwq187.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ST8 alpha-2,8-Sialyltransferase 8B/ST8SIA2 Antibody (AF6590)(1)

Publication using AF6590

• Tianxiang Huang, Lena H. Nguyen, Tiffany V. Lin, Xuan Gong, Longbo Zhang, Gi Bum Kim et al. In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons Neuroscience Research 2019-06-01 [PMID: 29857015] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ST8SIA2
• Uniprot:
  • Q92186()