Reg1 Antibody

Reg family proteins stimulated PSCs activation contributing to fibrosis in chronic pancreatitis. (a) Immunofluorescence analysis of alpha-smooth muscle actin ( alpha SMA, an activated PSCs marker, red) in the pancreas sections of indicated genotypes at 2 weeks, and 4 weeks (n = 3–4 mice). Nuclei were counterstained by DAPI (blue). (b) The percentage of epithelial cells with alpha SMA positive signals. Results represent mean ± SEM (n = 3–4 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. **P < 0.01 (c) Cropped images of western blot gels of Amylase, alpha SMA, Desmin, Reg1, Reg2, Reg3b of the pancreas of indicated genotypes at 0.5 day after birth(P0.5), 1w and 4w, p44/42 MAPK (ERK 1/2) and Akt were used as the loading control (n = 3 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels are presented in Supplementary Fig. 5–7. The right panel showed the respective densitometric quantification analysis of the relative intensity of alpha SMA. Densitometric quantification analysis of other proteins is presented in Supplementary Fig. 4.Results represent mean ± SEM (n = 3 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. ns: not significant; #P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reg family proteins stimulated PSCs activation contributing to fibrosis in chronic pancreatitis. (a) Immunofluorescence analysis of alpha-smooth muscle actin ( alpha SMA, an activated PSCs marker, red) in the pancreas sections of indicated genotypes at 2 weeks, and 4 weeks (n = 3–4 mice). Nuclei were counterstained by DAPI (blue). (b) The percentage of epithelial cells with alpha SMA positive signals. Results represent mean ± SEM (n = 3–4 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. **P < 0.01 (c) Cropped images of western blot gels of Amylase, alpha SMA, Desmin, Reg1, Reg2, Reg3b of the pancreas of indicated genotypes at 0.5 day after birth(P0.5), 1w and 4w, p44/42 MAPK (ERK 1/2) and Akt were used as the loading control (n = 3 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels are presented in Supplementary Fig. 5–7. The right panel showed the respective densitometric quantification analysis of the relative intensity of alpha SMA. Densitometric quantification analysis of other proteins is presented in Supplementary Fig. 4.Results represent mean ± SEM (n = 3 mice). Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison tests among four groups. ns: not significant; #P < 0.0001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reg deficiency led to the remission of chronic pancreatitis. (a) Representative pancreatic tissue section from mice of the indicated genotype at 8 weeks was stained with H&E or Azan (for collagen, dark blue) and Immunofluorescence staining of alpha-smooth muscle actin ( alpha SMA, an activated PSCs marker, red) (n = 5 mice). Scale bars: H&E, 50 μm; Azan, 100 μm (b) Pancreatic mRNA expression of proinflammatory cytokines interleukin 6(Il6), tumor necrosis factor a (Tnfa) and Il1b of indicated genotypes were assessed by qRT-PCR. The expression amounts of each gene were calculated relative to those of ribosomal protein S3 (Rps3) with the fold change to CP model_Reg+/+ mice. Mean ± SEM (n = 5 mice). Statistical analysis was performed by two-tailed unpaired Student t-test between two groups. *P < 0.05 (c) Cropped images of western blot gels of Amylase, alpha SMA and Reg1 of the pancreas of indicated genotypes at 8 weeks and Akt was used as the loading control (n = 5 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels and respective densitometric quantification analysis of the relative intensity of protein are presented in Supplementary Fig. 4,8. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reg deficiency led to the remission of chronic pancreatitis. (a) Representative pancreatic tissue section from mice of the indicated genotype at 8 weeks was stained with H&E or Azan (for collagen, dark blue) and Immunofluorescence staining of alpha-smooth muscle actin ( alpha SMA, an activated PSCs marker, red) (n = 5 mice). Scale bars: H&E, 50 μm; Azan, 100 μm (b) Pancreatic mRNA expression of proinflammatory cytokines interleukin 6(Il6), tumor necrosis factor a (Tnfa) and Il1b of indicated genotypes were assessed by qRT-PCR. The expression amounts of each gene were calculated relative to those of ribosomal protein S3 (Rps3) with the fold change to CP model_Reg+/+ mice. Mean ± SEM (n = 5 mice). Statistical analysis was performed by two-tailed unpaired Student t-test between two groups. *P < 0.05 (c) Cropped images of western blot gels of Amylase, alpha SMA and Reg1 of the pancreas of indicated genotypes at 8 weeks and Akt was used as the loading control (n = 5 mice). The samples derived from the same experiment and gels were processed in parallel. Images of the entire gels and respective densitometric quantification analysis of the relative intensity of protein are presented in Supplementary Fig. 4,8. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37500741), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Reg1 Antibody Summary

• Immunogen: E. coli-derived recombinant mouse Reg1
  Gln22-Gly165
  Accession # P43137
• Specificity: Detects mouse Reg1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse (rm) Reg2 is observed and less than 5% cross-reactivity with rmReg3A, recombinant human (rh) Reg1B, rhReg4, recombinant rat (rr) Reg3 and rrReg2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Reg1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Reg1 Antibody

• ICRF
• Islet cells regeneration factor
• Islet of Langerhans regenerating protein
• lithostathine-1-alpha
• Pancreatic stone protein
• pancreatic stone protein, secretory
• Pancreatic thread protein
• protein-X
• PSPregenerating islet-derived 1 alpha (pancreatic stone protein, pancreatic threadprotein)
• PSPS1REG-1-alpha
• PSPSMGC12447
• PTPP19
• Reg1
• regenerating islet-derived 1 alpha
• Regenerating islet-derived protein 1-alpha
• Regenerating protein I alpha
• REGlithostathine 1 alpha

Background

Reg1, also known as pancreatic thread protein (PTP), pancreatic stone protein (PSP) and lithostathine, is the founding member of the Reg family of proteins, which are secreted proteins with a C-type lectin domain. Reg1 is expressed during islet regeneration. Reg1 functions in an autocrine/paracrine fashion via a cell surface Reg receptor that mediates a growth signal for beta -cell regeneration (1).

1. Okamoto, H. and S. Takasawa (2002) Diabetes 51:5462.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Reg1 Antibody (AF1657)(3)

Publications using AF1657

• J Son, W Du, M Esposito, K Shariati, H Ding, Y Kang, D Accili Genetic and pharmacologic inhibition of ALDH1A3 as a treatment of beta-cell failure Nature Communications, 2023-02-02;14(1):558. 2023-02-02 [PMID: 36732513] (IHC, Mouse)
• T Saito, Y Tanaka, Y Morishita, K Ishibashi Proteomic analysis of AQP11-null kidney: Proximal tubular type polycystic kidney disease Biochem Biophys Rep, 2017-11-23;13(0):17-21. 2017-11-23 [PMID: 29204517] (Western Blot, Mouse)
• Zhong B, Strnad P, Toivola DM, Tao GZ, Ji X, Greenberg HB, Omary MB Reg-II is an exocrine pancreas injury-response product that is up-regulated by keratin absence or mutation. Mol. Biol. Cell, 2007-09-26;18(12):4969-78. 2007-09-26 [PMID: 17898082] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: Reg1
• Uniprot:
  • P43137()