PHF6 Antibody

Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Western Blot: PHF6 Antibody [NB100-68262] - Image from a customer review testing on 293T cells.

• Reactivity: Hu, Mu, Bv, Pm-Or
• Applications: WB, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PHF6 Antibody Summary

• Description: Novus Biologicals Rabbit PHF6 Antibody (NB100-68262) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-PHF6 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 315 and 365 of human PHD finger protein 6 (NP_115834.1).
• Predicted Species: Bovine (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Use in chromatin immunoprecipitation reported in scientific literature (PMID: 30551478). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID:32187544). PHF6 antibody validated for WB from a verified customer review.
• Theoretical MW: 41.29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Adipose Subcutaneous Whole Tissue Lysate (Adult Whole Diabetes)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30403997).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PHF6 Antibody

• BFLS
• BORJ
• Borjeson-Forssman-Lehmann syndrome
• KIAA1823
• MGC14797
• PHD finger protein 6
• PHD-like zinc finger protein

Background

This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHF6 Antibody (NB100-68262)(8)

Publications using NB100-68262

• Liu B, Wang J, Wu X et al. Regulation of stress susceptibility by chromatin-binding protein PHF6 in the pituitary intermediate lobe. Molecular psychiatry 2025-10-06 [PMID: 41053436]
• Dilan Rasool, Audrey Burban, Ahmad Sharanek, Ariel Madrigal, Jinghua Hu, Keqin Yan, Dianbo Qu, Anne K Voss, Ruth S Slack, Tim Thomas, Azad Bonni, David J Picketts, Vahab D Soleimani, Hamed S Najafabadi, Arezu Jahani-Asl PHF6-mediated transcriptional control of NSC via Ephrin receptors is impaired in the intellectual disability syndrome BFLS EMBO Reports 2024-03-01 [PMID: 38429579]
• Oh S, Boo K, Kim J et al. The chromatin-binding protein PHF6 functions as an E3 ubiquitin ligase of H2BK120 via H2BK12Ac recognition for activation of trophectodermal genes Nucleic Acids Res. 2020-07-31 [PMID: 32735658] (Chemotaxis, Mouse)
• Gan L, Sun J, Yang S et al. Chromatin-Binding Protein PHF6 Regulates Activity-Dependent Transcriptional Networks to Promote Hunger Response Cell Rep 2020-03-17 [PMID: 32187544] (IHC-Fr, Mouse)
• Warmerdam DO, Alonso-de Vega I, Wiegant WW et al. PHF6 promotes non-homologous end joining and G2 checkpoint recovery EMBO Rep. 2019-11-29 [PMID: 31782600] (Human)
• Xiang J, Wang G, Xia T, Chen Z. The depletion of PHF6 decreases the drug sensitivity of T-cell acute lymphoblastic leukemia to prednisolone. Biomedicine & Pharmacotherapy. 1905-07-11 [PMID: 30551478] (Chemotaxis, Human)
• Cheng C, Deng PY, Ikeuchi Y et al. Characterization of a Mouse Model of Borjeson-Forssman-Lehmann Syndrome. Cell Rep. 2018-11-06 [PMID: 30403997] (WB, Mouse)
• Meacham CE, Lawton LN, Soto-Feliciano YM et al. A genome-scale in vivo loss-of-function screen identifies Phf6 as a lineage-specific regulator of leukemia cell growth. Genes Dev 2015-03-01 [PMID: 25737277] (WB, Human)

Review for PHF6 Antibody (NB100-68262) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/05/2013

Summary

• Application: Western Blot
• Sample Tested: 293T whole cell lysate prepared with Cell Signal Lysis buffer
• Species: Human

FAQs for PHF6 Antibody (NB100-68262). (Showing 1 - 2 of 2 FAQs).

1. I read on the webpage that the C terminal is raised by human protein 315-365 amino acid. I am wondering which tag did you guys use to make the recombinant protein as the antigen?
   • Unfortunately, those details are part of our production process and we are not able to disclose the information.
2. Could you please check whether the recombinant protein is conjugated with reactive immunogens as well (like KLH) ?
   • Unfortunately, those details are part of our production process and we are not able to disclose the information.

Bioinformatics

• Gene Symbol: PHF6
• Entrez:
  • 84295(Human)
• Uniprot:
  • Q8IWS0(Human)