PHF6 Antibody

Images

 
Independent Antibodies: Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.
Immunohistochemistry: PHF6 Antibody [NB100-68262] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: PHF6 Antibody [NB100-68262] - Image from a customer review testing on 293T cells.

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

PHF6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 315 and 365 of human PHD finger protein 6 (NP_115834.1).
Predicted Species
Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunohistochemistry 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Theoretical MW
41.29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Adipose Subcutaneous Lysate (NB820-59281)
PHF6 Lysate (NBP2-64796)
Reviewed Applications
Read 1 Review rated 5
using
NB100-68262 in the following applications:

Publications
Read Publication using
NB100-68262 in the following applications:

  • WB
    1 publication

Reactivity Notes

Based on 100% sequence identity, this antibody is predicted to react with Orangutan.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PHF6 Antibody

  • BFLS
  • BORJ
  • Borjeson-Forssman-Lehmann syndrome
  • KIAA1823
  • MGC14797
  • PHD finger protein 6
  • PHD-like zinc finger protein

Background

This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PHF6 Antibody (NB100-68262)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for PHF6 Antibody (NB100-68262) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-68262:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot PHF6 NB100-68262
Enlarge
reviewed by:
Anonymous
WB Human 12/05/2013
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Summary

ApplicationWestern Blot
Sample Tested293T whole cell lysate prepared with Cell Signal Lysis buffer
SpeciesHuman

Blocking

Blocking Details5% skim milk powder dissolved in TBS-T, for one hour at Room Temperature

Primary Anitbody

Dilution Ratio1:1000 dilution in 5% skim milk powder dissolved in TBS-T. Placed in small sealed bag, incubated overnight at 4oC with rotation.

Secondary Antibody

Secondary DescriptionAnti-Rabbit 1:5000 dilution in 5% skim milk TBS-T buffer
Secondary Manufacturer Cat#Amersham (GE healthcare)
Secondary Concentration1:5000 dilution

Details

Detection NotesDetection with LAS-3000 Fuji detection system. Used the Femto detection reagent from Pierce.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PHF6 Antibody (NB100-68262). (Showing 1 - 2 of 2 FAQ).

  1. I read on the webpage that the C terminal is raised by human protein 315-365 amino acid. I am wondering which tag did you guys use to make the recombinant protein as the antigen?
    • Unfortunately, those details are part of our production process and we are not able to disclose the information.
  2. Could you please check whether the recombinant protein is conjugated with reactive immunogens as well (like KLH) ?
    • Unfortunately, those details are part of our production process and we are not able to disclose the information.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on PHF6

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Recent Reviews

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1
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Anonymous
12/05/2013
Application: WB
Species: Human

Bioinformatics

Gene Symbol PHF6
Entrez
Uniprot