PHF6 Antibodies
We offer PHF6 Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Sandwich ELISA, Western Blot. Each PHF6 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our PHF6 Antibodies can be used in a variety of model species: Human, Mouse. Use the list below to choose the PHF6 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our PHF6 polyclonal antibodies and browse our PHF6 monoclonal antibody catalog.
Alternate Names for PHF6 Antibodies
anti-PHF6 antibody, anti-BFLS antibody, anti-BORJ antibody, anti-Borjeson-Forssman-Lehmann syndrome antibody, anti-KIAA1823 antibody, anti-MGC14797 antibody, anti-PHD finger protein 6 antibody, anti-PHD-like zinc finger protein antibody
9 Results for "phf6" in Primary Antibodies
![Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68262-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-NB100-68262-img0006.jpg)
![Western Blot: PHF6 Antibody [NB100-68262] - Image from a customer review testing on 293T cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68262-img0003.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining in human epididymis and liver tissues using anti-PHF6 antibody. Corresponding PHF6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining in human epididymis and liver tissues using anti-PHF6 antibody. Corresponding PHF6 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PHF6 Antibody [NBP1-88646] - Analysis in human cell line EFO-21.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NBP1-88646-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: PHF6 Antibody [NBP1-88646] - Staining of human cell line U-251MG shows positivity in nucleus and nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88646-img0004.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0017.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human epididymis shows high expression.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0010.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human tonsil shows strong nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0014.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human cerebral cortex shows moderate nuclear and nucleolar positivity in neurons.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0015.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP1-88646] - Staining of human testis shows strong nuclear positivity in Leydig cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP1-88646-img0016.jpg)
![Immunohistochemistry: PHF6 Antibody (PSH02-35) [NBP3-32746] - Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-PHF6 antibody (NBP3-32746) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32746) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32746_rabbit-phf6-mab-psh02-35-298202413585573.jpg "Immunohistochemistry: PHF6 Antibody (PSH02-35) [NBP3-32746] - Immunohistochemical analysis of paraffin-embedded human ovary tissue with Rabbit anti-PHF6 antibody (NBP3-32746) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32746) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: PHF6 Antibody (PSH02-35) [NBP3-32746] - Immunocytochemistry analysis of NIH/3T3 cells labeling PHF6 with Rabbit anti-PHF6 antibody (NBP3-32746) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PHF6 antibody (NBP3-32746) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32746_rabbit-phf6-mab-psh02-35-29202410334086.jpg)
![Western Blot: PHF6 Antibody (PSH02-35) [NBP3-32746] - Western blot analysis of PHF6 on different lysates with Rabbit anti-PHF6 antibody (NBP3-32746) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: HEK-293 cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Jurkat cell lysate (20 µg/Lane) Lane 5: A431 cell lysate (20 µg/Lane) Lane 6: HepG2 cell lysate (20 µg/Lane) Lane 7: MCF7 cell lysate (20 µg/Lane) Lane 8: COS-1 cell lysate (20 µg/Lane) Lane 9: NIH/3T3 cell lysate (20 µg/Lane) Lane 10: Neuro-2a cell lysate (20 µg/Lane) Lane 11: RAW264.7 cell lysate (20 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32746_rabbit-phf6-mab-psh02-35-3920248485021.jpg)
![Western Blot: PHF6 Antibody [NB100-68261] - Detection of Human PHF6 on HeLa whole cell lysate using NB100-68261. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68262.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68261-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68261] - Detection of Human PHF6 on HeLa whole cell lysate using NB100-68261. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68262.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68261] - Section of human breast carcinoma. Affinity purified rabbit anti- PHF6 used at 1:200 (1 ug/mL). Detection by DAB.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-NB100-68261-img0005.jpg)
![Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68260-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68260] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NB100-68260-img0001.jpg)
![Western Blot: PHF6 Antibody [NBP2-19799] - MCF-7 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF6 antibody [N2C2], Internal (NBP2-19799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19799_rabbit-polyclonal-phf6-antibody-91020242013409.jpg "Western Blot: PHF6 Antibody [NBP2-19799] - MCF-7 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF6 antibody [N2C2], Internal (NBP2-19799) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PHF6 Antibody [NBP2-19799] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19799-img0002.jpg)
![Western Blot: PHF6 Antibody (8F24) [H00084295-M01-100ug] - Detection against Immunogen (36.63 KDa) .](http://images.novusbio.com/fullsize/PHF6-Antibody-8F24-Western-Blot-H00084295-M01-100ug-img0002.jpg "Western Blot: PHF6 Antibody (8F24) [H00084295-M01-100ug] - Detection against Immunogen (36.63 KDa) .")
![ELISA: PHF6 Antibody (8F24) [H00084295-M01-100ug] - Detection limit for recombinant GST tagged PHF6 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PHF6-Antibody-8F24-ELISA-H00084295-M01-100ug-img0001.jpg)
![Immunohistochemistry-Paraffin: PHF6 Antibody [NBP2-97699] - Immunochemical staining of human PHF6 in human epididymis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-Paraffin-NBP2-97699-img0001.jpg "Immunohistochemistry-Paraffin: PHF6 Antibody [NBP2-97699] - Immunochemical staining of human PHF6 in human epididymis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.")