PHF6 Antibody

Western Blot: PHF6 Antibody [NB100-68261] - Detection of Human PHF6 on HeLa whole cell lysate using NB100-68261. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68262.

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68261] - Section of human breast carcinoma. Affinity purified rabbit anti- PHF6 used at 1:200 (1 ug/mL). Detection by DAB.

• Reactivity: Hu, Bv, Pm
• Applications: WB, IHC, IP, Single-Cell Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PHF6 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 125 and 175 of human PHD finger protein 6 using the numbering given in entry NP_115834.1 (GeneID 84295).
• Predicted Species: Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2 - 5 ug/mg lysate
  • Single Cell Western 100 ug/mL
  • Western Blot 1:2000 - 1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Single Cell Western reported by an internal validation on treated IMR32 cells at a concentration of 100ug/ml

Reactivity Notes

Orangutan (100%).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PHF6 Antibody

• BFLS
• BORJ
• Borjeson-Forssman-Lehmann syndrome
• KIAA1823
• MGC14797
• PHD finger protein 6
• PHD-like zinc finger protein

Background

This gene is a member of the plant homeodomain (PHD)like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PHF6 Antibody (NB100-68261)(1)

Publication using NB100-68261

• Frederick Rehfeld, Daniel Maticzka, Sabine Grosser, Pina Knauff, Murat Eravci, Imre Vida, Rolf Backofen, F. Gregory Wulczyn The RNA-binding protein ARPP21 controls dendritic branching by functionally opposing the miRNA it hosts Nature Communications 2018-03-26 [PMID: 29581509]

Bioinformatics

• Gene Symbol: PHF6
• Entrez:
  • 84295(Human)
  • 70998(Mouse)
• Uniprot:
  • Q8IWS0(Human)