PHF6 Antibody

Western Blot: PHF6 Antibody [NB100-68260] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-PHF6 antibody NB100-68260 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68261 and NB100-68262, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68260] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

PHF6 Antibody Summary

• Description: Novus Biologicals Rabbit PHF6 Antibody (NB100-68260) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human PHD finger protein 6 using the numbering given in entry NP_115834.1 (GeneID 84295).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PHF6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PHF6 Antibody

• BFLS
• BORJ
• Borjeson-Forssman-Lehmann syndrome
• KIAA1823
• MGC14797
• PHD finger protein 6
• PHD-like zinc finger protein

Background

This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PHF6
• Entrez:
  • 84295(Human)
• Uniprot:
  • Q8IWS0(Human)