Phenylalanine Hydroxylase Antibody

Images

 
Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - (0.1ug/ml) staining of Mouse (A) and Rat (B) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Phenylalanine Hydroxylase Antibody [NBP1-52084] - Analysis of Phenylalanine Hydroxylase Human Liver lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

Phenylalanine Hydroxylase Antibody Summary

Immunogen
Peptide with sequence C-ESRPSRLKKDE corresponding to internal region according to NP_000268.1.
Epitope
C-ESRPSRLKKDE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PAH
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 48 kDa band observed in human liver lysates and approx. 52 kDa band in mouse and rat liver lysates (calculated MW of 51.9 kDa band according to NP_000268.1).
Publications
Read Publication using NBP1-52084.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Phenylalanine Hydroxylase Antibody

  • EC 1.14.16
  • EC 1.14.16.1
  • PH
  • phe-4-monooxygenase
  • phenylalanine 4-monooxygenase
  • phenylalanine hydroxylase
  • phenylalanine-4-hydroxylase
  • PKU
  • PKU1

Background

The PAH gene encodes the enzyme phenylalanine hydroxylase (PAH), which converts phenylalanine to tyrosine and is the rate-limiting enzyme in phenylalanine catabolism. Mammalian PAH is a soluble, homotetrameric protein which is abundantly expressed in human liver. Deficiency of PAH activity results in the autosomal recessive disorder phenylketonuria (PKU), which is characterized by mental retardation unless a low phenylalanine diet is introduced early in life. The PAH gene, which maps to human chromosome 12q23.2, contains all the genetic information necessary to code for functional PAH, demonstrating that a single gene is involved in the classic disease phenotype. Numerous mutations can impair the PAH gene, which result in decreased enzyme activity and give rise to varying degrees of PKU. Multiple isozymes of PAH have been reported to exist, but these are most likely allelic variants of PAH that produce protein subunits with slightly different charge and electrophoretic migration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-02300
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-74398
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NB100-128
Species: Hu, Mu, Rt
Applications: ChIP, GS, ICC/IF, IHC, IHC-P
H00009771-B01P
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB300-109
Species: Hu, Mu, Rt, Dr, I, Ma
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
NBP2-04611
Species: Hu
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
H00009356-M05
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
H00003347-M01
Species: Hu
Applications: ELISA, ICC/IF
MAB1106
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP2-07799
Species: Hu
AF8038
Species: Hu, Mu, Rt
Applications: WB
NBP2-24722
Species: Hu
Applications: WB, IHC-P
AF5725
Species: Hu
Applications: WB, IHC
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE

Publications for Phenylalanine Hydroxylase Antibody (NBP1-52084)(1)

Reviews for Phenylalanine Hydroxylase Antibody (NBP1-52084) (0)

There are no reviews for Phenylalanine Hydroxylase Antibody (NBP1-52084). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Phenylalanine Hydroxylase Antibody (NBP1-52084) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Phenylalanine Hydroxylase Products

Bioinformatics Tool for Phenylalanine Hydroxylase Antibody (NBP1-52084)

Discover related pathways, diseases and genes to Phenylalanine Hydroxylase Antibody (NBP1-52084). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Phenylalanine Hydroxylase Antibody (NBP1-52084)

Discover more about diseases related to Phenylalanine Hydroxylase Antibody (NBP1-52084).
 

Pathways for Phenylalanine Hydroxylase Antibody (NBP1-52084)

View related products by pathway.

PTMs for Phenylalanine Hydroxylase Antibody (NBP1-52084)

Learn more about PTMs related to Phenylalanine Hydroxylase Antibody (NBP1-52084).

Blogs on Phenylalanine Hydroxylase

There are no specific blogs for Phenylalanine Hydroxylase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Phenylalanine Hydroxylase Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol PAH
Entrez
Uniprot