PHD4/HIF Prolyl Hydroxylase 4 Antibody - Azide and BSA Free

Western Blot: PHD4/HIF Prolyl Hydroxylase 4 Antibody [H00054681-D01P] - Analysis of P4HTM expression in transfected 293T cell line by P4HTM polyclonal antibody.Lane 1: P4HTM transfected lysate(63.10 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PHD4/HIF Prolyl Hydroxylase 4 Antibody - Azide and BSA Free Summary

• Immunogen: P4HTM (NP_808807.2, 1 a.a. - 563 a.a.) full-length human protein. MAAAAVTGQRPETAAAEEASRPQWAPPDHCQAQAAAGLGDGEDAPVRPLCKPRGICSRAYFLVLMVFVHLYLGNVLALLLFVHYSNGDESSDPGPQHRAQGPGPEPTLGPLTRLEGIKVGHERKVQLVTDRDHFIRTLSLKPLLFEIPGFLTDEECRLIIHLAQMKGLQRSQILPTEEYEEAMSTMQVSQLDLFRLLDQNRDGHLQLREVLAQTRLGNGWWMTPESIQEMYAAIKADPDGDGVLSLQEFSNMDLRDFHKYMRSHKAESSELVRNSHHTWLYQGEGAHHIMRAIRQRVLRLTRLSPEIVELSEPLQVVRYGEGGHYHAHVDSGPVYPETICSHTKLVANESVPFETSCRQVSPNWGLPSILRPGTPMTQAQPCTVGVPLGMGPGDHWVIPVSPWEHPQLGTCSVPPLPYSYMTVLFYLNNVTGGGETVFPVADNRTYDEMSLIQDDVDLRDTRRHCDKGNLRVKPQQGTAVFWYNYLPDGQGWVGDVDDYSLHGGCLVTRGTKWIANNWINVDPSRARQALFQQEMARLAREGGTDSQPEWALDRAYRDARVEL
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: P4HTM
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is useful for Western Blot, Functional

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein G purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PHD4/HIF Prolyl Hydroxylase 4 Antibody - Azide and BSA Free

• EC 1.14.11.-
• EGLN4
• HIFPH4
• HIF-PH4
• HIF-prolyl hydroxylase 4
• HPH-4
• hypoxia-inducible factor prolyl 4-hydroxylase
• Hypoxia-inducible factor prolyl hydroxylase 4
• P4H with transmembrane domain
• P4H-TMFLJ20262
• PH4 hypoxia inducible factor prolyl 4 hydroxylase
• PH-4
• PHD4
• proline 4-hydroxylase
• prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum)
• Prolyl hydroxlase domain-containing 4
• transmembrane prolyl 4-hydroxylase

Background

Prolyl hydroxylase 4 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the prolyl hydroxylase sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. Prolyl hydroxylase is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, prolyl hydroxylases convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. Prolyl hydroxylase 4, specifically, catalyzes the post-translational formation of 4-hydroxyproline in HIF alpha proteins. It may function as a cellular oxygen sensor and, under normoxic conditions, may targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: P4HTM
• Uniprot:
  • NP_808807.2(Human)