PGM1 Antibody (AT84G2) - BSA Free

Western Blot: PGM1 Antibody (AT84G2) [NBP3-12868] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP3-12868 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP. Lane 1: 293T cell lysate. Lane 2: HepG2 cell lysate. Lane 3: NIH/3T3 cell lysate. Lane 4: Jurkat cell lysate. Lane 5: HeLa cell lysate. Lane 6: U87MG cell lysate

Immunocytochemistry/Immunofluorescence: PGM1 Antibody (AT84G2) [NBP3-12868] - HeLa cells were stained with NBP3-12868 (1:100). Alexa Fluor 488-conjugated secondary antibody was used (green). DAPI was stained the cell nucleus (blue).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: AT84G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PGM1 Antibody (AT84G2) - BSA Free Summary

• Immunogen: Recombinant human PGM1 (1-562aa) purified from E. coli (Accession number: NP_002624)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PGM1 Antibody (AT84G2) - BSA Free

• EC 5.4.2
• EC 5.4.2.2
• Glucose phosphomutase 1
• GSD14
• PGM 1
• phosphoglucomutase 1
• phosphoglucomutase-1

Background

Phosphoglucomutases (PGM; EC 5.4.2.2) catalyze the transfer of phosphate between the 1 and 6 positions of glucose. Isozymes of PGM are monomeric, with molecular masses of about 60 kD, and are encoded by several genes, including PGM1. In most cell types, PGM1 isozymes predominate, representing about 90% of total PGM activity. One exception is red cells, where PGM2 (MIM 172000) is a major isozyme (Putt et al., 1993 (PubMed 8257433)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGM1