PGAM2 Antibody (5A7) - BSA Free

Western Blot: PGAM2 Antibody (5A7) [NBP2-59410] - Lane 1: Recombinant protein (50ng), Lane 2: HeLa cell lysates, Lane 3: HepG2 cell lysates, Lane 4: 293T cell lysates, Lane 5: Jurkat cell lysates, Lane 6: NIH3T3 cell lysates, Lane 7: A549 cell lysates, Lane 8: MCF7 cell lysates

Immunocytochemistry/Immunofluorescence: PGAM2 Antibody (5A7) [NBP2-59410] - Analysis of PGAM2 in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 5A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PGAM2 Antibody (5A7) - BSA Free Summary

• Description: Novus Biologicals Mouse PGAM2 Antibody (5A7) - BSA Free (NBP2-59410) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PGAM2 (1-253aa) purified from E. coli
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PGAM2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PGAM2 Antibody (5A7) - BSA Free

• BPG-dependent PGAM 2
• EC 3.1.3.13
• EC 5.4.2.1
• EC 5.4.2.4
• Muscle-specific phosphoglycerate mutase
• PGAM-MGSD10
• PGAMMMGC88743
• phosphoglycerate mutase 2 (muscle)
• phosphoglycerate mutase 2
• Phosphoglycerate mutase isozyme M

Background

Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PGAM2