PRKAG3 Antibody (2C7A7) - BSA Free

Western Blot: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis using PRKAG3 mAb against human PRKAG3 (AA: 9-151) recombinant protein. (Expected MW is 41.1 kDa)

Immunocytochemistry/Immunofluorescence: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis of Hela cells using PRKAG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Immunohistochemistry: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis of paraffin-embedded rectum tissues using PRKAG3 mouse mAb with DAB staining.

Western Blot: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis using PRKAG3 mAb against HEK293 (1) and PRKAG3 (AA: 9-151)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2C7A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PRKAG3 Antibody (2C7A7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PRKAG3 (AA: 9-151) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRKAG3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 54.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PRKAG3 Antibody (2C7A7) - BSA Free

• 5'-AMP-activated protein kinase subunit gamma-3
• AMPK gamma 3
• AMPK gamma-3 chain
• AMPK gamma3
• AMPK subunit gamma-3
• AMPKG3
• AMPKG3,5'-AMP-activated protein kinase, gamma-3 subunit
• PRKAG3
• protein kinase, AMP-activated, gamma 3 non-catalytic subunit

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKAG3