PRKAG3 Antibody (2C7A7) - BSA Free

Images

 
Western Blot: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis using PRKAG3 mAb against human PRKAG3 (AA: 9-151) recombinant protein. (Expected MW is 41.1 kDa)
Immunocytochemistry/ Immunofluorescence: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis of Hela cells using PRKAG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis of paraffin-embedded rectum tissues using PRKAG3 mouse mAb with DAB staining.
Western Blot: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Analysis using PRKAG3 mAb against HEK293 (1) and PRKAG3 (AA: 9-151)-hIgGFc transfected HEK293 (2) cell lysate.
ELISA: PRKAG3 Antibody (2C7A7) [NBP2-61796] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
2C7A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

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Catalog# & Formulation Size Price

PRKAG3 Antibody (2C7A7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human PRKAG3 (AA: 9-151) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PRKAG3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Theoretical MW
54.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PRKAG3 Antibody (2C7A7) - BSA Free

  • 5'-AMP-activated protein kinase subunit gamma-3
  • AMPK gamma 3
  • AMPK gamma-3 chain
  • AMPK gamma3
  • AMPK subunit gamma-3
  • AMPKG3
  • AMPKG3,5'-AMP-activated protein kinase, gamma-3 subunit
  • PRKAG3
  • protein kinase, AMP-activated, gamma 3 non-catalytic subunit

Background

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. It is dominantly expressed in skeletal muscle. Studies of the pig counterpart suggest that this subunit may play a key role in the regulation of energy metabolism in skeletal muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKAG3