PGAM1/2/4 Antibody

Images

 
Western Blot: PGAM1/2/4 Antibody [NB100-774] - staining of Mouse (A), Rat (B) and Pig (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Subcellular localization of PGAM with the use of antibody directed to C-terminal peptide of the proteinThe localization was examined in cultures ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Localization of PGAM with antibody directed to the C-terminal peptide of the protein -in nuclei of KLN-205 cells ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

PGAM1/2/4 Antibody Summary

Immunogen
Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1.
Specificity
Please note this antibody is expected to recognize the products of 3 highly similar genes.
Predicted Species
Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PGAM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Peptide ELISA Detection limit 1:28000
  • Western Blot 0.05-0.15 ug/ml
Application Notes
WB: Approx 27kDa band observed in Human Cerebellum and in Human and Mouse Liver lysates, and approx. 28kDa band observed in Rat and Pig Liver lysates (calculated MW of 28.8kDa according to Human NP_002620.1, NP_000281.2, and NP_001025062.1, Mouse NP_075907.2, Rat NP_445742.2 and Pig XP_003483583.1 ).
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
PGAM1 Overexpression Lysate
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using NB100-774.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PGAM1/2/4 Antibody

  • PGAM1
  • PGAMA
  • PGAM-B
  • phosphoglycerate mutase 1

Background

PGAM1 is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM1 in mammals, a muscle and brain isoform. PGAM1 has also been linked to non-spherocytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PGAM1/2/4 Antibody (NB100-774)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PGAM1/2/4 Antibody (NB100-774) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional PGAM1/2/4 Products

Array NB100-774

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Bioinformatics

Gene Symbol PGAM1
Entrez
Uniprot