Western Blot: PGAM1/2/4 Antibody [NB100-774] - staining of Mouse (A), Rat (B) and Pig (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Subcellular localization of PGAM with the use of antibody directed to C-terminal peptide of the proteinThe localization was examined in cultures ...read more
Biological Strategies: Immunocytochemistry/ Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Localization of PGAM with antibody directed to the C-terminal peptide of the protein -in nuclei of KLN-205 cells ...read more
WB: Approx 27kDa band observed in Human Cerebellum and in Human and Mouse Liver lysates, and approx. 28kDa band observed in Rat and Pig Liver lysates (calculated MW of 28.8kDa according to Human NP_002620.1, NP_000281.2, and NP_001025062.1, Mouse NP_075907.2, Rat NP_445742.2 and Pig XP_003483583.1 ).
29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for PGAM1/2/4 Antibody
phosphoglycerate mutase 1
PGAM1 is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM1 in mammals, a muscle and brain isoform. PGAM1 has also been linked to non-spherocytic anemia.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our PGAM1/2/4 Antibody and receive a gift card or discount.