PGAM1/2/4 Antibody

Western Blot: PGAM1/2/4 Antibody [NB100-774] - staining of Mouse (A), Rat (B) and Pig (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Subcellular localization of PGAM with the use of antibody directed to C-terminal peptide of the proteinThe localization was examined in cultures of non-small cell lung carcinoma (NSCLC) HL-1 cardiomyocytes, astrocytes and in breast cancer tissue section (BC). Arrows point nucleoli. Bar=5 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.4044), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: PGAM1/2/4 Antibody [NB100-774] - Localization of PGAM with antibody directed to the C-terminal peptide of the protein -in nuclei of KLN-205 cells treated with drugs disturbing ribosomal biogenesisActD = actinomycin D; PI - propidium iodide. Bar=5 um. Image collected and cropped by CiteAb from the following publication (oncotarget.com/lookup/doi/10.18632/oncotarget.4044), licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PGAM1/2/4 Antibody Summary

• Immunogen: Peptide with sequence C-KAMEAVAAQGKAKK, from the C Terminus of the protein sequence according to NP_002620.1; NP_000281.2; NP_001025062.1.
• Specificity: Please note this antibody is expected to recognize the products of 3 highly similar genes.
• Predicted Species: Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PGAM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Peptide ELISA Detection limit 1:28000
  • Western Blot 0.05-0.15 ug/ml
• Application Notes: WB: Approx 27kDa band observed in Human Cerebellum and in Human and Mouse Liver lysates, and approx. 28kDa band observed in Rat and Pig Liver lysates (calculated MW of 28.8kDa according to Human NP_002620.1, NP_000281.2, and NP_001025062.1, Mouse NP_075907.2, Rat NP_445742.2 and Pig XP_003483583.1 ).
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)
  PGAM1 Overexpression Lysate
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PGAM1/2/4 Antibody

• PGAM1
• PGAMA
• PGAM-B
• phosphoglycerate mutase 1

Background

PGAM1 is an enzyme that catalyzes 3 reactions; the isomerization of 2-PGA to 3-PGA with 2,3-DPG as a primer, the synthesis of 2,3-DPG from 1,3-DPG with 3-PGA as a primer and the degradation of 2,3-DPG to 3-PGA. There are two different isoforms of PGAM1 in mammals, a muscle and brain isoform. PGAM1 has also been linked to non-spherocytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PGAM1/2/4 Antibody (NB100-774)(4)

Publications using NB100-774

• Gizak A, McCubrey JA, Rakus D. Cell-to-cell lactate shuttle operates in heart and is important in age-related heart failure Aging (Albany NY) 2020-02-08 [PMID: 32035422]
• Gizak A, Grenda M, Mamczur P et al. Insulin/IGF1-PI3K-dependent nucleolar localization of a glycolytic enzyme--phosphoglycerate mutase 2, is necessary for proper structure of nucleolus and RNA synthesis. Oncotarget 2015-07-10 [PMID: 26033454]
• Chaneton B, Hillmann P, Zheng L et al. Serine is a natural ligand and allosteric activator of pyruvate kinase M2 Nature 2012-10-14 [PMID: 23064226] (Human)
• Sakoda S, Shanske S, DiMauro S, Schon EA. Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family. J Biol Chem 1988-11-15 [PMID: 2846553]

Bioinformatics

• Gene Symbol: PGAM1
• Entrez:
  • 5223(Human)
• Uniprot:
  • NP_002620(Human)