PFKP Antibody (OTI1D6)

Images

 
Genetic Strategies: Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cellsand PFKP-Knockout HeLa cells were separated by SDS-PAGE and ...read more
Immunocytochemistry/ Immunofluorescence: PFKP Antibody (OTI1D6) [NBP2-01539] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PFKP.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
Flow Cytometry: PFKP Antibody (1D6) [NBP2-01539] - Analysis of Jurkat cells, using anti-PFKP antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: PFKP Antibody (1D6) [NBP2-01539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFKP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Western Blot: PFKP Antibody (1D6) [NBP2-01539] Analysis of extracts (35ug) from 9 different cell lines by using anti-PFKP monoclonal antibody.
Western Blot: PFKP Antibody (OTI1D6) [NBP2-01539] - Analysis of extracts (10ug) from 9 Human tissues by using anti-PFKP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: ...read more
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PFKP mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PFKP Antibody (1D6) [NBP2-01539] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-PFKP mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, KO
Clone
OTI1D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.48 mg/ml
Validated by:
     

Genetic Strategies

   

Order Details

PFKP Antibody (OTI1D6) Summary

Immunogen
Full length human recombinant protein of human PFKP(NP_002618) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
PFKP
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:150
  • Knockout Validated
Theoretical MW
85.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
PFKP Knockout HeLa Cell Lysate

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.48 mg/ml
Purity
Affinity purified

Alternate Names for PFKP Antibody (OTI1D6)

  • 6-phosphofructokinase type C
  • 6-phosphofructokinase, platelet type
  • EC 2.7.1
  • EC 2.7.1.11
  • PFK-CFLJ40226
  • PFKFplatelet type
  • Phosphofructokinase 1
  • phosphofructokinase, platelet
  • phosphohexokinase

Background

The PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM; MIM 610681) and liver (PFKL; MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PFKP Antibody (NBP2-01539) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-73344AF350
Alexa Fluor 405 NBP2-73344AF405
Alexa Fluor 488 NBP2-73344AF488
Alexa Fluor 532 NBP2-73344AF532
Alexa Fluor 594 NBP2-73344AF594
Alexa Fluor 647 NBP2-73344AF647
Alexa Fluor 700 NBP2-73344AF700
Alexa Fluor 750 NBP2-73344AF750
Allophycocyanin NBP2-73344APC
Allophycocyanin/Cy7 NBP2-73344APCCY7
Biotin NBP2-73344B
DyLight 350 NBP2-73344UV
DyLight 405 NBP2-73344V
DyLight 488 NBP2-73344G
DyLight 550 NBP2-73344R
DyLight 594 NBP2-73344DL594
DyLight 650 NBP2-73344C
DyLight 680 NBP2-73344FR
DyLight 755 NBP2-73344IR
FITC NBP2-73344F
HRP NBP2-73344H
Janelia Fluor 549 NBP2-73344JF549
Janelia Fluor 646 NBP2-73344JF646
PE NBP2-73344PE
PE/Atto594 NBP2-73344PEATT594
PE/Cy5.5 NBP2-73344PECY55
PE/Cy7 NBP2-73344PECY7
PerCP NBP2-73344PCP

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Bioinformatics

Gene Symbol PFKP
Entrez