Peroxiredoxin 4 Antibody - Azide and BSA Free

Western Blot: Peroxiredoxin 4 Antibody [NBP2-99498] - Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell Lysate Lane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:31 kDa

Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP2-99498] - Immunochemical staining of human Peroxiredoxin 4 in human testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Peroxiredoxin 4 Antibody [NBP2-99498] - Immunochemical staining of human Peroxiredoxin 4 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Peroxiredoxin 4 Antibody [NBP2-99498] - Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-PRDX4 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody:Anti-PRDX4 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 31 kDaObserved band size :28 kDa

Immunocytochemistry/Immunofluorescence: Peroxiredoxin 4 Antibody [NBP2-99498] - Immunofluorescence staining of Peroxiredoxin 4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99498 antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Peroxiredoxin 4 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Peroxiredoxin 4.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRDX4
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Peroxiredoxin 4 Antibody - Azide and BSA Free

• Antioxidant enzyme AOE372
• AOE372
• AOE37-2PRX-4
• EC 1.11.1.15
• Peroxiredoxin 4
• Peroxiredoxin IV
• peroxiredoxin-4
• PRDX4
• Prx4
• prx-4
• prx-IV
• thioredoxin peroxidase (antioxidant enzyme)
• Thioredoxin peroxidase AO372
• Thioredoxin-dependent peroxide reductase A0372

Background

Peroxiredoxin-4 is encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRDX4