Pentraxin 3/TSG-14 Antibody (5B7)

Western Blot: Pentraxin 3/TSG-14 Antibody (5B7) [H00005806-M01] - PTX3 monoclonal antibody (M01), clone 5B7. Analysis of PTX3 expression in NIH/3T3.

Western Blot: Pentraxin 3/TSG-14 Antibody (5B7) [H00005806-M01] - PTX3 monoclonal antibody (M01), clone 5B7. Analysis of PTX3 expression in PC-12.

Sandwich ELISA: Pentraxin 3/TSG-14 Antibody (5B7) [H00005806-M01] - Detection limit for recombinant GST tagged PTX3 is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Pentraxin 3/TSG-14 Antibody (5B7) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PTX3 (NP_002843, 282 a.a. ~ 380 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SLWVNGELAATTVEMATGHIVPEGGILQIGQEKNGCCVGGGFDETLAFSGRLTGFNIWDSVLSNEEIRETGGAESCHIRGNIVGWGVTEIQPHGGAQYV
• Localization: Secreted
• Specificity: PTX3 - pentraxin-related gene, rapidly induced by IL-1 beta
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTX3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Pentraxin 3/TSG-14 Antibody (5B7)

• alpha-induced protein 5
• pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor
• Pentaxin-related protein PTX3
• Pentraxin 3
• pentraxin 3, long
• pentraxin-3
• pentraxin-related gene, rapidly induced by IL-1 beta
• pentraxin-related protein PTX3
• PTX3
• TNF alpha-induced protein 5
• TNFAIP5
• TSG14
• TSG-14
• TSG-14pentaxin-related gene, rapidly induced by IL-1 beta
• Tumor necrosis factor alpha-induced protein 5
• tumor necrosis factor, alpha-induced protein 5
• Tumor necrosis factor-inducible gene 14 protein
• tumor necrosis factor-inducible protein TSG-14

Background

Pentraxin 3 (PTX3) is the prototypic member of the long pentraxin family sharing the C-terminal domain with short pentraxins (C-reactive protein and serum amyloid P component) and containing a unique N-terminal domain. The protein is a soluble pattern recognition receptor and represents a nonredundant component of the humoral innate immunity against selected pathogens. PTX3 is rapidly produced and released at inflammatory sites by diverse cell types including monocytes/macrophages, endothelial cells, vascular smooth muscle cells, fibroblasts, and adipocytes. It interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens. PTX3 has been implicated in vascular damage, angiogenesis, atherosclerosis, and restenosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pentraxin 3/TSG-14 Antibody (H00005806-M01)(1)

Publication using H00005806-M01

• Qin S, Chen X, Gao M et al. Prenatal Exposure to Lipopolysaccharide Induces PTX3 Expression and Results in Obesity in Mouse Offspring. Inflammation 2017-08-02 [PMID: 28770376]

Bioinformatics

• Gene Symbol: PTX3
• Entrez:
  • 5806(Human)
• OMIM:
  • 602492(Human)
• Uniprot:
  • NP_002843()