Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

ELISA: Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric) [NBP2-69890] - Samples were spiked with high concentrations of Rat PEDFR/PNPLA2/ATGL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric) [NBP2-69890] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat ATGL in samples. No significant cross-reactivity or interference between Rat ATGL and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.83%
• Intra-Assay: CV% < 4.32%
• Spike Recovery: 86-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: PNPLA2

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat PEDFR/PNPLA2/ATGL ELISA Kit (Colorimetric)

• Adipose triglyceride lipase
• ATGL
• ATGL1110001C14Rik
• Calcium-independent phospholipase A2
• Desnutrin
• EC 3.1.1.3
• FP17548
• IPLA2-zeta
• patatin-like phospholipase domain containing 2
• patatin-like phospholipase domain-containing protein 2
• patatin-like phospholipase domain-containing protein 2-like
• PEDF R
• PEDFR
• PEDF-R
• Pigment epithelium-derived factor
• PNPLA2
• Transport-secretion protein 2
• transport-secretion protein 2.2
• triglyceride hydrolase
• TTS2
• TTS2.2
• TTS-2.2
• TTS2DKFZp667M109

Background

Patatin-like phospholipase domain-containing protein 2 (PNPLA2), also known as adipose triglyceride lipase (ATGL), is a lipolytic enzyme required for mobilization of fatty acids from triglyceride stores in adipose tissue. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. However, ATGL has now been shown to catalyze the initial step of triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets. Thus, ATGL and HSL coordinate to catabolize stored triglycerides in adipose tissue of mammals.

Defects in PNPLA2 can lead to neutral lipid storage disease with myopathy (NLSDM), a neutral lipid storage disorder with myopathy but without ichthyosis.

ATGL antibodies can serve as useful tools for adipocyte studies and research on triglyceride processing and fat storage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: PNPLA2