PEDFR/PNPLA2/ATGL Antibody

Western Blot: ATGL Antibody [NB110-41536] - Detection of ATGL in human adipose tissue lysate.

Immunocytochemistry/Immunofluorescence: ATGL Antibody [NB110-41536] - ATGL antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Discontinued

PEDFR/PNPLA2/ATGL Antibody Summary

• Immunogen: A synthetic peptide within the C-terminal region (residues 400-500) of human ATGL. [Accession# NP_005171]
• Localization: Cytoplasm, Adiposomes
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PNPLA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:1000-1:2000
  • Western Blot 1:1000
• Application Notes: This ATGL antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In ICC/IF, cytoplasmic/adiposomal staining was observed in HeLa cells.
• Control:

  PEDFR/PNPLA2/ATGL Overexpression Lysate

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PEDFR/PNPLA2/ATGL Antibody

• Adipose triglyceride lipase
• ATGL
• ATGL1110001C14Rik
• Calcium-independent phospholipase A2
• Desnutrin
• EC 3.1.1.3
• FP17548
• IPLA2-zeta
• patatin-like phospholipase domain containing 2
• patatin-like phospholipase domain-containing protein 2
• patatin-like phospholipase domain-containing protein 2-like
• PEDF R
• PEDFR
• PEDF-R
• Pigment epithelium-derived factor
• PNPLA2
• Transport-secretion protein 2
• transport-secretion protein 2.2
• triglyceride hydrolase
• TTS2
• TTS2.2
• TTS-2.2
• TTS2DKFZp667M109

Background

ATGL (adipose triglyceride lipase) is the rate-limiting lipolytic enzyme which catalyzes initial step of triglyceride (TAG) hydrolysis in adipocytes as well as non-adipocyte lipid droplets. Among adipose lipases (ATGL, hormone-sensitive lipase/HSL and monoacylglycerol lipase), HSL is a diacylglycerol lipase, whereas ATGL prefers TAG substrates. CGI-58 coactivates ATGL's reactions and G0/G1 switch gene 2 exerts supressive effects on ATGL. Localized in lipid droplet and cell membrane as single-pass type II membrane protein, ATGL is mainly expressed in adipose tissue, however, has also been reported in cardiac/skeletal muscles, gastrointestinal tract, normal retina and retinoblastoma. ATGL is inhibited by BEL and is activated by ABHD5 as well as SERPINF1, and is induced during differentiation of primary preadipocytes to adipocytes with expression increasing from fetal to adult in RPE cells. Besides TAG hydrolysis, ATGL has acylglycerol transacylase activity also and can act coordinately with LIPE/HLS within the lipolytic cascade, regulate size of adiposome and implicate in adiposomes degradation. ATGL implicates in energy homeostasis/response to starvation by enhancing TAGs hydrolysis and providing free fatty acids to be oxidized in situations of energy depletion. ATGL genetic variations have been linked with risk of NIDDM and neutral lipid storage disease with myopathy (NLSDM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PEDFR/PNPLA2/ATGL Antibody (NB110-41536)(1)

Publication using NB110-41536

• Hu Lijuan, Cui Rui, Liu Hongyi, Wang Feng. Emodin and rhein decrease levels of hypoxia-inducible factor-1a in human pancreatic cancer cells and attenuate cancer cachexia in athymic mice carrying these cells. Oncotarget 2017-01-01 [PMID: 29152137] (WB, Mouse)

Bioinformatics

• Gene Symbol: PNPLA2