PEDFR/PNPLA2/ATGL Antibody

Images

 
Western Blot: ATGL Antibody [NB110-41536] - Detection of ATGL in human adipose tissue lysate.
Immunocytochemistry/ Immunofluorescence: ATGL Antibody [NB110-41536] - ATGL antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PEDFR/PNPLA2/ATGL Antibody Summary

Immunogen
A synthetic peptide within the C-terminal region (residues 400-500) of human ATGL. [Accession# NP_005171]
Localization
Cytoplasm, Adiposomes
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PNPLA2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:1000-1:2000
Application Notes
This ATGL antibody is useful for Western Blot and Immunocytochemistry/Immunofluorescence. In ICC/IF, cytoplasmic/adiposomal staining was observed in HeLa cells.
Control
PEDFR/PNPLA2/ATGL Overexpression Lysate
Control Peptide
PEDFR/PNPLA2/ATGL Protein (NB110-41536PEP)
Publications
Read Publication using
NB110-41536 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PEDFR/PNPLA2/ATGL Antibody

  • Adipose triglyceride lipase
  • ATGL
  • ATGL1110001C14Rik
  • Calcium-independent phospholipase A2
  • Desnutrin
  • EC 3.1.1.3
  • FP17548
  • IPLA2-zeta
  • patatin-like phospholipase domain containing 2
  • patatin-like phospholipase domain-containing protein 2
  • patatin-like phospholipase domain-containing protein 2-like
  • PEDF R
  • PEDFR
  • PEDF-R
  • Pigment epithelium-derived factor
  • PNPLA2
  • Transport-secretion protein 2
  • transport-secretion protein 2.2
  • triglyceride hydrolase
  • TTS2
  • TTS2.2
  • TTS-2.2
  • TTS2DKFZp667M109

Background

ATGL (adipose triglyceride lipase) is the rate-limiting lipolytic enzyme which catalyzes initial step of triglyceride (TAG) hydrolysis in adipocytes as well as non-adipocyte lipid droplets. Among adipose lipases (ATGL, hormone-sensitive lipase/HSL and monoacylglycerol lipase), HSL is a diacylglycerol lipase, whereas ATGL prefers TAG substrates. CGI-58 coactivates ATGL's reactions and G0/G1 switch gene 2 exerts supressive effects on ATGL. Localized in lipid droplet and cell membrane as single-pass type II membrane protein, ATGL is mainly expressed in adipose tissue, however, has also been reported in cardiac/skeletal muscles, gastrointestinal tract, normal retina and retinoblastoma. ATGL is inhibited by BEL and is activated by ABHD5 as well as SERPINF1, and is induced during differentiation of primary preadipocytes to adipocytes with expression increasing from fetal to adult in RPE cells. Besides TAG hydrolysis, ATGL has acylglycerol transacylase activity also and can act coordinately with LIPE/HLS within the lipolytic cascade, regulate size of adiposome and implicate in adiposomes degradation. ATGL implicates in energy homeostasis/response to starvation by enhancing TAGs hydrolysis and providing free fatty acids to be oxidized in situations of energy depletion. ATGL genetic variations have been linked with risk of NIDDM and neutral lipid storage disease with myopathy (NLSDM).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PEDFR/PNPLA2/ATGL Antibody (NB110-41536)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for PEDFR/PNPLA2/ATGL Antibody (NB110-41536): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PEDFR/PNPLA2/ATGL Antibody (NB110-41536) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-41536AF350
Alexa Fluor 405 NB110-41536AF405
Alexa Fluor 488 NB110-41536AF488
Alexa Fluor 532 NB110-41536AF532
Alexa Fluor 594 NB110-41536AF594
Alexa Fluor 647 NB110-41536AF647
Alexa Fluor 700 NB110-41536AF700
Alexa Fluor 750 NB110-41536AF750
Biotin NB110-41536B
DyLight 350 NB110-41536UV
DyLight 405 NB110-41536V
DyLight 488 NB110-41536G
DyLight 550 NB110-41536R
DyLight 594 NB110-41536DL594
DyLight 650 NB110-41536C
DyLight 680 NB110-41536FR
DyLight 755 NB110-41536IR
FITC NB110-41536F
HRP NB110-41536H
Janelia Fluor 549 NB110-41536JF549
Janelia Fluor 646 NB110-41536JF646

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Bioinformatics

Gene Symbol PNPLA2