PDX-1/IPF1 Antibody [Alexa Fluor® 700]

• Reactivity: Mu, Rt
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

PDX-1/IPF1 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant mouse PDX‑1/IPF1
  Met1-Arg284
  Accession # P52946
• Specificity: Detects mouse PDX‑1/IPF1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDX-1/IPF1 Antibody [Alexa Fluor® 700]

• Glucose-sensitive factor
• IDX1
• IDX-1GSF
• Insulin promoter factor 1
• insulin promoter factor 1, homeodomain transcription factor
• Insulin upstream factor 1
• IPF1
• IPF1pancreas/duodenum homeobox protein 1
• Islet/duodenum homeobox-1
• MODY4IUF1
• pancreatic and duodenal homeobox 1
• pancreatic-duodenal homeobox factor 1
• PDX-1
• PDX-1IPF-1
• somatostatin transcription factor 1
• Somatostatin-transactivating factor 1
• STF-1IUF-1

Background

PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42 kDa member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta -cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta -cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284 amino acids (aa) in length (Accession # P52946). It contains one transactivation domain (aa 13‑73) a PBX1 P-P-M-W-K dimerization motif (aa 119‑124), and a DNA-binding homeodomain with an embedded NLS (aa 148‑206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.