PDK3 Antibody - BSA Free

Images

 
Western Blot: PDK3 Antibody [NBP2-56356] - Western blot analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: PDK3 Antibody [NBP2-56356] - Staining of human cell line MCF7 shows localization to nucleoli & mitochondria.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free

Order Details

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Catalog# & Formulation Size Price

PDK3 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit PDK3 Antibody - BSA Free (NBP2-56356) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: SAWRHYKTTPEADDWSNPSSEPRDASKYKAKQDKIKTNRTF
Predicted Species
Mouse (98%), Rat (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK3
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PDK3 Recombinant Protein Antigen (NBP2-56356PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for PDK3 Antibody - BSA Free

  • EC 2.7.11.2
  • PDHK3
  • PDK3
  • Pyruvate dehydrogenase kinase isoform 3
  • pyruvate dehydrogenase kinase, isoenzyme 3
  • pyruvate dehydrogenase kinase, isozyme 3
  • pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial

Background

PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDK3