PDK3 Antibody

Western Blot: PDK3 Antibody [NBP1-32581] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581] - Ca922 xenograft, using PDK3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: PDK3 Antibody [NBP1-32581] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:500.

Western Blot: PDK3 Antibody [NBP1-32581] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:1000.

Immunofluorescence analysis of methanol-fixed HepG2, using PDK3(NBP1-32581) antibody at 1:200 dilution.

• Reactivity: Hu, Mu, Sq, Xp, Rt, Bv, Ch
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PDK3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Predicted Species: Rat (96%), Chicken (93%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Frog (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for PDK3 Antibody

• EC 2.7.11.2
• PDHK3
• PDK3
• Pyruvate dehydrogenase kinase isoform 3
• pyruvate dehydrogenase kinase, isoenzyme 3
• pyruvate dehydrogenase kinase, isozyme 3
• pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial

Background

PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDK3 Antibody (NBP1-32581)(5)

Publications using NBP1-32581

• Yang ES, Do Y, Cheon SY et al. Andrographolide suppresses aerobic glycolysis and induces apoptotic cell death by inhibiting pyruvate dehydrogenase kinase 1 expression Oncology reports 2023-04-01 [PMID: 36825595] (WB, Human)
  
  Details:
  Dilution used in WB 1:000 in 5% skim milk
• �korja Mili? N, Dolinar K, Mi� K et al. Suppression of Pyruvate Dehydrogenase Kinase by Dichloroacetate in Cancer and Skeletal Muscle Cells Is Isoform Specific and Partially Independent of HIF-1? International Journal of Molecular Sciences 2021-08-10 [PMID: 34445316] (Human)
• Schoonjans CA, Joudiou N, Brusa D et al. Acidosis-induced metabolic reprogramming in tumor cells enhances the anti-proliferative activity of the PDK inhibitor dichloroacetate Cancer Lett. 2020-02-01 [PMID: 31812695] (WB, Human)
• Hou X, Zhang L, Han L et al. Differential function of pyruvate dehydrogenase kinases during mouse oocyte maturation. J. Cell. Sci. 2015-05-19 [PMID: 25991547] (ICC/IF, Mouse)
• Ho N, Coomber BL. Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells. Exp Cell Res 2015-02-01 [PMID: 25536473] (Human)

Review for PDK3 Antibody (NBP1-32581) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/22/2015

Summary

• Application: Western Blot
• Sample Tested: Human cancer cell whole cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: PDK3
• Entrez:
  • 5165(Human)
• Uniprot:
  • Q15120(Human)