PDK3 Antibody

Images

 
Western Blot: PDK3 Antibody [NBP1-32581] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PDK3 antibody [N1C2] diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: PDK3 Antibody [NBP1-32581] - Methanol-fixed HepG2, using PDK3(NBP1-32581) antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PDK3 Antibody [NBP1-32581] - Paraffin-embedded Ca922 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

PDK3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human PDK3. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Rat (96%), Bovine (98%), Chicken (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-32581 in the following applications:

Publications
Read Publications using
NBP1-32581 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25991547).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PDK3 Antibody

  • EC 2.7.11.2
  • Pyruvate dehydrogenase kinase isoform 3
  • pyruvate dehydrogenase kinase, isoenzyme 3
  • pyruvate dehydrogenase kinase, isozyme 3
  • pyruvate dehydrogenase, lipoamide, kinase isozyme 3, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDK3 Antibody (NBP1-32581)(2)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
(1)
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Human
(1)
Mouse
(1)
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Review for PDK3 Antibody (NBP1-32581) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-32581:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 08/22/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesHuman

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:1000, overnight

Secondary Antibody

Secondary DescriptionDonkey anti-rabbit IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA934V
Secondary Concentration1:2000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDK3 Antibody (NBP1-32581) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PDK3 Antibody (NBP1-32581)

Discover related pathways, diseases and genes to PDK3 Antibody (NBP1-32581). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on PDK3

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Recent Reviews

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4
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2
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1
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Anonymous
08/22/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol PDK3
Entrez
Uniprot