PDIR Antibody

Images

 
Western Blot: PDIR Antibody [NBP1-92252] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/ Immunofluorescence: PDIR Antibody [NBP1-92252] - Staining of human cell line U-251 MG shows positivity in nucleoli, nucleus but not nucleoli and golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry: PDIR Antibody [NBP1-92252] - Immunohistochemistry analysis in human placenta and skeletal muscle tissues using NBP1-92252 antibody. Corresponding PDIA5 RNA-seq data are presented for the same ...read more
Immunohistochemistry: PDIR Antibody [NBP1-92252] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic and decidual cells.
Immunohistochemistry: PDIR Antibody [NBP1-92252] - Staining of human testis shows moderate to strong granular cytoplasmic positivity Leydig cells and in cells in seminiferous ducts.
Immunohistochemistry: PDIR Antibody [NBP1-92252] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry: PDIR Antibody [NBP1-92252] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.
Independent Antibodies: Staining of human liver, placenta, skeletal muscle and testis using NBP1-92252 (A) shows similar protein distribution across tissues to independent antibody NBP1-92250 (B).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
 

Independent Antibodies

       

Order Details

PDIR Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VTFKSIVAFLKDPKGPPLWEEDPGAKDVVHLDSEKDFRRLLKKEEKPLLIMFYAPWCSMCKRMMPHFQKAATQLRGHAVLAGMNVYSSEFE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDIA5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1.0 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
PDIR Protein (NBP1-92252PEP)
Publications
Read Publication using
NBP1-92252 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for PDIR Antibody

  • FLJ30401
  • PDIREC 5.3.4.1
  • protein disulfide isomerase family A, member 5
  • protein disulfide isomerase-associated 5
  • Protein disulfide isomerase-related protein
  • protein disulfide-isomerase A5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDIR Antibody (NBP1-92252)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDIR Antibody (NBP1-92252) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on PDIR

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Bioinformatics

Gene Symbol PDIA5