PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

Immunohistochemistry-Paraffin: PDGF Receptor alpha Antibody (RM0004-3G28) [NB110-61020] - PDGFR-a immunohistochemistry staining of paraffin sections of mouse pancreas from LPS exposed animals.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: RM0004-3G28
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free (NB110-61020) is a monoclonal antibody validated for use in IHC and WB. Anti-PDGFR alpha Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Mouse PDGFR alpha (extracellular domain)
• Specificity: This antibody recognizes mouse PDGF receptor alpha in western blot. No cross reactivity to mouse PDGF receptor beta.
• Isotype: IgG2
• Clonality: Monoclonal
• Host: Rat
• Gene: PDGFRA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:400-1:800
• Application Notes: For immunohistochemsitry, it is critical to use a mouse IgG preabsorbed secondary antibody.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for PDGFR alpha Antibody (RM0004-3G28) - Azide and BSA Free

• alpha-type platelet-derived growth factor receptor
• CD140 antigen-like family member A
• CD140a antigen
• CD140a
• EC 2.7.10
• EC 2.7.10.1
• MGC74795
• PDGF R alpha
• PDGFR alpha
• PDGFR2
• PDGFRA
• PDGFRA/BCR fusion
• PDGF-R-alpha
• platelet-derived growth factor receptor, alpha polypeptide
• rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein
• RHEPDGFRA

Background

Platelet derived growth factor receptor (PDGF Receptor) is a transmembrane glycoprotein of 170-185 kDa which undergoes homo or heterodimerization into complexes of alpha and beta subunits upon ligand binding, depending on the isoform of PDGF (PDGF AA, BB or AB) that binds. The phosphorylation of tyrosine residues in the now activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras, Raf and ERK 1 and 2 pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGFR alpha Antibody (NB110-61020)(1)

Publication using NB110-61020

• Egners A, Rezaei M, Kuzmanov A et al. PHD3 Acts as Tumor Suppressor in Mouse Osteosarcoma and Influences Tumor Vascularization via PDGF-C Signaling Cancers (Basel) 2018-12-06 [PMID: 30563292]

Bioinformatics

• Gene Symbol: PDGFRA
• Entrez:
  • 18595(Mouse)
• Uniprot:
  • P26618()