PDGF R beta Antibody [Unconjugated]

Images

 
Recombinant Human PDGF R beta Fc Chimera (Catalog # 385-PR) inhibits Recombinant Human PDGF-BB (Catalog # 220‑BB) induced proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange ...read more
PDGF R beta was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 15 µg/mL Goat Anti-Human PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF385) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

PDGF R beta Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human PDGF R beta
Leu33-Phe530 (Glu241Asp)
Accession # P09619
Specificity
Detects human PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PDGF R beta is observed, and less than 1% cross-reactivity with recombinant human PDGF sR alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PDGFRB
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Neutralization 10-40 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF385 in the following applications:

Publications
Read Publications using
AF385 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R beta Antibody [Unconjugated]

  • beta-type platelet-derived growth factor receptor
  • CD140 antigen-like family member B
  • CD140b antigen
  • CD140b
  • EC 2.7.10
  • EC 2.7.10.1
  • JTK12
  • PDGF R beta
  • PDGFR
  • PDGFR1
  • PDGFRB
  • PDGF-R-beta
  • platelet-derived growth factor receptor, beta polypeptide

Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF‑AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt-3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PDGF R beta Antibody (AF385)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 5 applications: ICC, IHC, IHC Frozen, Neut, WB.


Filter By Application
ICC
(2)
IHC
(2)
IHC Frozen
(1)
Neut
(6)
WB
(4)
All Applications
Filter By Species
Human
(12)
Mouse
(1)
Rabbit
(2)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using AF385 Applications Species
SE Ha, MY Lee, M Kurahashi, L Wei, BG Jorgensen, C Park, PJ Park, D Redelman, KC Sasse, LS Becker, KM Sanders, S Ro Transcriptome analysis of PDGFR?+ cells identifies T-type Ca2+ channel CACNA1G as a new pathological marker for PDGFR?+ cell hyperplasia PLoS ONE, 2017;12(8):e0182265. 2017 [PMID: 28806761] (IHC Frozen,, Human) IHC Frozen Human
EN Schock, JN Struve, CF Chang, TJ Williams, J Snedeker, AC Attia, RW Stottmann, SA Brugmann A tissue-specific role for intraflagellar transport genes during craniofacial development PLoS ONE, 2017;12(3):e0174206. 2017 [PMID: 28346501] (IHC,, Mouse) IHC Mouse
Rizvi S, Mertens J, Bronk S, Hirsova P, Dai H, Roberts L, Kaufmann S, Gores G Platelet-derived growth factor primes cancer-associated fibroblasts for apoptosis. J Biol Chem, 2014;289(33):22835-49. 2014 [PMID: 24973208] (WB,, Human) WB Human
Yuan, Liangpin, Zhang, Hongying, Liu, Jingbo, Rubin, Joshua B, Cho, Yoon-Jae, Shu, Hui Kuo, Schniederjan, Matthew, MacDonald, Tobey J Growth factor receptor-Src-mediated suppression of GRK6 dysregulates CXCR4 signaling and promotes medulloblastoma migration. Mol Cancer, 2013;12(0):18. 2013 [PMID: 23497290] (Neut,, Human) Neut Human
PDGFRbeta expression and function in fibroblasts derived from pluripotent cells is linked to DNA demethylation. J. Cell. Sci., 2012;125(0):2276-87. 2012 [PMID: 22344267] (Neut,, Human) Neut Human
Zemskov EA, Loukinova E, Mikhailenko I, Coleman RA, Strickland DK, Belkin AM Regulation of platelet-derived growth factor receptor function by integrin-associated cell surface transglutaminase. J. Biol. Chem., 2009;284(24):16693-703. 2009 [PMID: 19386600] (WB,, Human) WB Human
Miyake T, Aoki M, Morishita R Inhibition of anastomotic intimal hyperplasia using a chimeric decoy strategy against NFkappaB and E2F in a rabbit model. Cardiovasc. Res., 2008;79(4):706-14. 2008 [PMID: 18515844] (WB,, Rabbit) WB Rabbit
Vonlaufen A, Joshi S, Qu C, Phillips PA, Xu Z, Parker NR, Toi CS, Pirola RC, Wilson JS, Goldstein D, Apte MV Pancreatic stellate cells: partners in crime with pancreatic cancer cells. Cancer Res., 2008;68(7):2085-93. 2008 [PMID: 18381413] (ICC,, Human) ICC Human
Li R, Maminishkis A, Wang FE, Miller SS PDGF-C and -D induced proliferation/migration of human RPE is abolished by inflammatory cytokines. Invest. Ophthalmol. Vis. Sci., 2007;48(12):5722-32. 2007 [PMID: 18055825] (Neut,, Human) Neut Human
Ball SG, Shuttleworth CA, Kielty CM Vascular endothelial growth factor can signal through platelet-derived growth factor receptors. J. Cell Biol., 2007;177(3):489-500. 2007 [PMID: 17470632] (Neut,, Human) Neut Human
Show All 15 Publications.

Reviews for PDGF R beta Antibody (AF385) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 application: IHC-P, IF.

Reviews using AF385:
Filter by Applications
IHC-P
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23126372
SpeciesHuman
 
reviewed by:
Anonymous
IF Human 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23126372
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23126372

Details

Detection NotesSee PMID 23126372

Comments

CommentsPublished: See PMID 23126372Independent review from antibodyresource.com

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: IHC-P
Species: Human

 
Anonymous
01/08/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol PDGFRB
Entrez
Uniprot