PDGF R beta Antibody (61520)

• Reactivity: Hu
• Applications: WB
• Clone: 61520
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

PDGF R beta Antibody (61520) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human PDGF R beta
  Leu33-Lys531 (Lys531Glu)
  Accession # P09619
• Specificity: Detects human PDGF R beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant mouse (rm) PDGF R beta , rhPDGF R alpha , rhgp130, or rhIL-6 R.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRB
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PDGF R beta Antibody (61520)

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF‑BB, and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta  are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF, and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist.

1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PDGF R beta Antibody (MAB385)(3)

Publications using MAB385

• MG Jeong, K Zhou, S Park, H An, Y Kwon, Y Chang, DH Kim, SH Ryu Analysis of transient membrane protein interactions by single-molecule diffusional mobility shift assay Experimental & Molecular Medicine, 2021-02-19;0(0):. 2021-02-19 [PMID: 33603128] (ICC, Human)
• Melanie D. Sweeney, Abhay P. Sagare, Maricarmen Pachicano, Michael G. Harrington, Elizabeth Joe, Helena C. Chui et al. A novel sensitive assay for detection of a biomarker of pericyte injury in cerebrospinal fluid Alzheimer's & Dementia 2020-06-01 [PMID: 32301266]
• Bone HK, Nelson AS, Goldring CE, Tosh D, Welham MJ A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. J. Cell. Sci., 2011-05-24;124(0):1992-2000. 2011-05-24 [PMID: 21610099] (ICC, Human)

Bioinformatics

• Gene Symbol: PDGFRB
• Uniprot:
  • P09619()