PDGF R beta Antibody (2G7B7) - BSA Free

Western Blot: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis using PDGFRB mAb against HEK293 (1) and PDGFRB (AA: 33-133)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis of NIH/3T3 cells using PDGFRB mouse mAb (green) and negative control (red).

Western Blot: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Analysis using PDGFRB mAb against human PDGFRB (AA: 33-133) recombinant protein. (Expected MW is 37 kDa).

ELISA: PDGF R beta Antibody (2G7B7) [NBP2-52529] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 2G7B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PDGF R beta Antibody (2G7B7) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human PDGF R beta (AA: 33-133) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDGFRB
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 124 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for PDGF R beta Antibody (2G7B7) - BSA Free

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-alpha and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDGFRB