Recombinant Rat PDGF-BB Protein

SDS-Page: Rat PDGF-BB Protein [NBP2-35285]

• Reactivity: Rt
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Rat PDGF-BB Protein Summary

• Description: A disulfide-linked homodimeric protein containing two 109 amino acid polypeptide (B chain) chains corresponding to PDGF-BB Source: E. coli

  Uniprot ID: Q05028

  Amino Acid Sequence: SLGSLAAAEP AVIAECKTRT EVFQISRNLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPVFKKATVT LEDHLACKCE TVVTPRPVT

• Details of Functionality: PDGF-BB Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PDGFB
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of PDGF-BB as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 24.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 1 x PBS, pH 6.9.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Rat PDGF-BB Protein

• c-sis;IBGC5;PDGF2;PDGF-2;PDGFB;Platelet-derived growth factor subunit B;SIS;SSV
• PDGFBB
• PDGF-BB

Background

Platelet-derived growth factor is a dimeric glycoprotein composed of two A (-AA) or two B (-BB) chains or a combination of the two (-AB). All PDGFs function as secreted, disulphide-linked homodimers, but only PDGFA and B can form functional heterodimers. PDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent in wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints in order to divide. Recombinant rat PDGF-AA contains 109 amino acids and has a molecular mass of 12.2 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for PDGF-BB Recombinant Protein (NBP2-35285)(1)

Publication using NBP2-35285

• Li C, Tan Yh, Sun J et Al. PAX6 contributes to the activation and proliferation of hepatic stellate cells via activating Hedgehog/GLI1 pathway Biochem. Biophys. Res. Commun. 2020-03-21 [PMID: 32209258]

FAQs for PDGF-BB Recombinant Protein (NBP2-35285). (Showing 1 - 1 of 1 FAQ).

1. I purchased the Rat PDGF-BB protein, but I cannot find the specificity. Does it cross-react only with RAT or also with other species?
   • Regarding the specificity of the active protein, the homology of this amino acid sequence is quite high among rats and other rodents~98-100% and with primates there is about ~89% homology. Therefore, one might assume that you could use this active protein in assays for some animals other than rats. I am not sure what the parameters are for your assay are but if you tell me more about it, I might be able to provide more specific information.  If you need an antibody to this protein, here are 3 antibodies to the human protein that have not been validated in any other species.

Bioinformatics

• Gene Symbol: PDGFB