PDCD10 Antibody

Images

 
Western Blot: PDCD10 Antibody [NBP1-47195] - K.O. = knock out (completely deleted) K.D. = knock down (not completely deleted) OV. = over expressed in COS1 cells. Figure 2. Western Analysis of anti-human CCM-3.
Immunocytochemistry/ Immunofluorescence: PDCD10 Antibody [NBP1-47195] - (green) of human foreskin (cryosection of unfixed tissue) with anti-human CCM3 (K5548; dilution 1:50) A) Note specific staining in the epidermis ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

PDCD10 Antibody Summary

Immunogen
highly pure full length recombinant human PDCD10
Specificity
The polyclonal antibody will detect recombinant human CCM-3 in WB and native CCM-3 in IHC
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PDCD10
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Western blotting: Use at 1-5 micrograms/milliliter IHC: The antibody has been tested for immunohistochemistry-paraffin

Reactivity Notes

The polyclonal antibody will detect recombinant human CCM-3

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Reconstitute with 0.1 ml sterilized water to a final concentration of 0.1 mg/ml.

Alternate Names for PDCD10 Antibody

  • apoptosis-related protein 15
  • cerebral cavernous malformations 3
  • MGC1212
  • MGC24477
  • programmed cell death 10
  • programmed cell death protein 10
  • TF-1 cell apoptosis-related protein 15
  • TFAR15CCM3Cerebral cavernous malformations 3 protein

Background

This gene encodes an evolutionarily conserved protein associated with cell apoptosis. The protein interacts with the serine/threonine protein kinase MST4 to modulate the extracellular signal-regulated kinase (ERK) pathway. It also interacts with and is phosphoryated by serine/threonine kinase 25, and is thought to function in a signaling pathway essential for vascular developent. Mutations in this gene are one cause of cerebral cavernous malformations, which are vascular malformations that cause seizures and cerebral hemorrhages. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PDCD10 Antibody (NBP1-47195) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PDCD10