PTPN13/PTPL1 Antibody

Images

 
Western Blot: PTPN13/PTPL1 Antibody [NB100-56139] - Analysis of PTPN13/PTPL1 using this antibody. In ovarian carcinoma cell lines PTPN13/PTPL1 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. ...read more
Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56139] - Analysis of PTPN13/PTPL1 in cell lines using this antibody at 1:2000. In ovarian carcinoma cell lines PTPN13/PTPL1 expression was detected in SK-OV-3 ...read more
Immunohistochemistry-Paraffin: PTPN13/PTPL1 Antibody [NB100-56139] - Ovarian carcinoma cores from a tissue microarray at 1:2000. A-D, samples are from four different patients. A1-D1 are high magnification images from ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PTPN13/PTPL1 Antibody Summary

Immunogen
A recombinant protein corresponding to amino acids 1279 to 1883 of human FAP-1 protein was used as immunogen; GenBank no. NP_542414.1.
Specificity
FAP-1 typically migrates at ~270 kDa on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights. The actual molecular weight of FAP-1 observed depends on the isoform expression.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PTPN13
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500-1:3000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
FAP-1 typically migrates at ~270 kDa on western blots. Thus for SDS-PAGE and western blot, the researcher should use techniques optimized for the transfer of proteins with high molecular weights.
Publications
Read Publications using
NB100-56139 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for PTPN13/PTPL1 Antibody

  • EAP-1
  • FAP-1
  • hPTPE1
  • PNP1
  • protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associatedphosphatase)
  • protein-tyrosine phosphatase 1, Fas-associated
  • PTP1E
  • PTP1EDKFZp686J1497
  • PTP-BAS
  • PTP-BASEC 3.1.3.48
  • PTP-BL
  • PTP-E1
  • PTPL1
  • PTPL1nonreceptor type 13
  • PTPLE
  • PTPN13
  • tyrosine-protein phosphatase non-receptor type 13

Background

FAP-1 (also known as Fas-associated phosphatase-1, PTPN13, PTP-BAS, hPTPIE, and PTPL1) is a member of the protein tyrosine phosphatase (PTP) family (reviewed in Meinhold-Heerlein et al, 2001; Savaskan et al, 2005; Foehr et al, 2005; and Ivanov et al, 2006). PTPs are enzymes that catalyze the removal of a phosphate group attached to a tyrosine residue. Most intracellular signaling involves reversible phosphorylation events; therefore, PTPs are central to the dynamic regulation of signaling cascades that underlie cell functions. For example, PTPs play key roles in regulating cell growth, differentiation, proliferation, inflammation, and oncogenic transformation. PTPs are emerging as a promising class of signaling targets for diseases such as cancer, neurodegeneration, diabetes, and inflammation. A key challenge is to identify specific PTPs that are invovled in a disease process and develop therapeutics to modulate the PTP. FAP-1 phosphatase is thought to be important in the Fas signaling pathway. FAP-1 binds to the cytosolic tail of the Fas receptor (Apo1, CD95) and inhibits Fas-induced apoptosis. Increased FAP-1 protein levels in some tumor cell lines and tumor tissues correlates with resistance to Fas-mediated apoptosis. In general, FAP-1 expression has been found to be highest in cell lines and tissues that are relatively resistant to Fas-mediated apoptosis. Gene transfer-mediated elevations in FAP-1 partially abolished Fas-induced apoptosis in a T cell line which is consistent with an inhibitory effect of FAP-1 on Fas signal transductions. Additionally, FAP-1 expression correlates with Fas resistant in ovarian cancer cell lines and FAP-1 is commonly expression in ovarian cancers. Human Fas has a putative consensus tyrosine phosphorylation site (Tyrosine 275) suggesting that Fas surface expression or signaling may be regulated by phosphorylation. FAP-1 has been shown to directly bind to Fas and may dephosphorylate Fas as part of the down regulation of the apoptotic pathway. It is thought that development of therapeutics to inhibit FAP-1 may increase the ability of tumor cells with upregulated FAP-1 to undergo apoptosis. FAP-1 is a large approx. 270 kDa protein. Multiple alternatively spliced FAP-1 transcript variants which encode distinct proteins have been reported, including shorter forms. Please see Application Notes section for additional details on FAP-1 isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PTPN13/PTPL1 Antibody (NB100-56139)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PTPN13
Entrez
Uniprot