PD-1 Antibody (J116)

Images

 
Flow Cytometry: PD-1 Antibody (J116) [NBP1-43107] - Staining of human PD-1 transfected cells with anti-human Anti-Human CD279 (PD-1) FITC (left)or PE (right). Appropriate isotype controls were used (open histogram). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IP
Clone
J116
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PD-1 Antibody (J116) Summary

Immunogen
The immunogen for this antibody was PD1.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PDCD1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
The J116 antibody has been tested by flow cytometric analysis of human PD-1 transfected cells. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.
Publications
Read Publications using
NBP1-43107 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for PD-1 Antibody (J116)

  • CD279 antigen
  • CD279
  • hPD-1
  • PD-1
  • PD1hPD-l
  • PDCD1
  • programmed cell death 1
  • programmed cell death protein 1
  • Protein PD-1
  • SLEB2

Background

The J116 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predominantly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Binding of the J116 monoclonal antibody inhibits PD-1 signal transduction, however, it does not block binding of the ligand PD-L1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PD-1 Antibody (NBP1-43107)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: FLOW.


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FLOW
(1)
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Human
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for PD-1 Antibody (NBP1-43107) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 647 Labeled NBP1-43107AF647
Allophycocyanin Labeled NBP1-43107APC
DyLight 350 Labeled NBP1-43107UV
DyLight 405 Labeled NBP1-43107V
DyLight 488 Labeled NBP1-43107G
DyLight 680 Labeled NBP1-43107FR
DyLight 755 Labeled NBP1-43107IR
PE Labeled NBP1-43107PE
PerCP Labeled NBP1-43107PCP

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Bioinformatics

Gene Symbol PDCD1
Entrez