PD-1 Antibody (12A7D7) - BSA Free

Western Blot: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Western blot analysis of PD-1 in A-20 cell lysate with PD-1 antibody at 1 u/mL.

Immunohistochemistry: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 u/mL.

Immunohistochemistry: PD-1 Antibody (12A7D7) - BSA Free [NBP1-75519] - Immunohistochemistry of PD-1 in mouse thymus tissue with PD-1 antibody at 5 u/mL.

WB Validation in Human HeartLoading: 10 ug of human heart lysateAntibodies: Mouse Monoclonal PD-1 Antibody (12A7D7), 2 ug/mL , 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clone: 12A7D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PD-1 Antibody (12A7D7) - BSA Free Summary

• Immunogen: A ~150 amino acid recombinant protein from near the amino terminus of mouse PD-1. aa 22 - 170
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PDCD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PD-1 Antibody (12A7D7) - BSA Free

• CD279 antigen
• CD279
• hPD-1
• PD1
• PD-1
• PD1hPD-l
• PDCD1
• programmed cell death 1
• programmed cell death protein 1
• Protein PD-1
• SLEB2

Background

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of danger signals such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDCD1