PBR Antibody (SA90-03)

Western Blot: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of PBR on different cell lysates using anti-PBR antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: NIH/3T3 Lane 3: HepG2

Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - ICC staining of PBR in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary ant

Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PBR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m

Flow Cytometry: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of Hela cells with PBR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded human colon cancer tissue using anti-PBR antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded human kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded mouse kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded mouse testis tissue using anti-PBR antibody. Counter stained with hematoxylin.

Product Discontinued

PBR Antibody (SA90-03) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human PBR aa 120-169 / 169. (SwissProt: P30536 Human; SwissProt: P50637 Mouse)
• Localization: Mitochondrion membrane
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TSPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:400
  • Immunoprecipitation
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for PBR Antibody (SA90-03)

• benzodiazepine peripheral binding site
• BZRPDBI
• IBP
• MBRPBS
• mDRC
• Mitochondrial benzodiazepine receptor
• PBR
• PBRBPBS
• PBRS
• peripheral benzodiazepine receptor
• Peripheral-type benzodiazepine receptor
• pk18
• PKBSbenzodiazapine receptor (peripheral)
• PTBR
• translocator protein (18kDa)
• translocator protein
• TSPO

Background

Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSPO