![Western Blot: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of PBR on different cell lysates using anti-PBR antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: NIH/3T3 Lane 3: HepG2](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Western-Blot-NBP2-67293-img0007.jpg "Western Blot: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of PBR on different cell lysates using anti-PBR antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: NIH/3T3 Lane 3: HepG2")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, IP |
| Clone | SA90-03 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant Protein |
| Localization | Mitochondrion membrane |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TSPO |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 1%BSA, 40%Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
Diseases for PBR Antibody (NBP2-67293)Discover more about diseases related to PBR Antibody (NBP2-67293).
| Pathways for PBR Antibody (NBP2-67293)View related products by pathway.
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PTMs for PBR Antibody (NBP2-67293)Learn more about PTMs related to PBR Antibody (NBP2-67293).
| Research Areas for PBR Antibody (NBP2-67293)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TSPO |
![Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Immunocytochemistry-Immunofluorescence-NBP2-67293-img0008.jpg "Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded mouse testis tissue using anti-PBR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Immunohistochemistry-Paraffin-NBP2-67293-img0006.jpg "Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded mouse testis tissue using anti-PBR antibody. Counter stained with hematoxylin.")
![Flow Cytometry: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of Hela cells with PBR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Flow-Cytometry-NBP2-67293-img0001.jpg "Flow Cytometry: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of Hela cells with PBR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.")
![Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Immunocytochemistry-Immunofluorescence-NBP2-67293-img0002.jpg "Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/PBR Antibody (SA90-03)-Immunocytochemistry-Immunofluorescence-NBP2-67293-img0009.jpg "Immunocytochemistry/Immunofluorescence: PBR Antibody (SA90-03) [NBP2-67293] - Staining PBR in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded human colon cancer tissue using anti-PBR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Immunohistochemistry-Paraffin-NBP2-67293-img0003.jpg "Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded human colon cancer tissue using anti-PBR antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded human kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Immunohistochemistry-Paraffin-NBP2-67293-img0004.jpg "Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded human kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded mouse kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/PBR-Antibody-(SA90-03)-Immunohistochemistry-Paraffin-NBP2-67293-img0005.jpg "Immunohistochemistry-Paraffin: PBR Antibody (SA90-03) [NBP2-67293] - Analysis of paraffin-embedded mouse kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.")
![Western Blot: CD77 Synthase Antibody [NBP1-62583] - THP-1 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/images2/CD77 Synthase Antibody-Western Blot-NBP1-62583-img0002.jpg)
![Western Blot: ZAK Antibody (3G5) [H00051776-M03] - ZAK monoclonal antibody (M03), clone 3G5. Western Blot analysis of ZAK expression in human stomach.](http://images.novusbio.com/images2/ZAK-Antibody-(3G5)-Western-Blot-H00051776-M03-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: ZAK Antibody (3G5) [H00051776-M03] - Analysis of monoclonal antibody to ZAK on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/images2/ZAK-Antibody-(3G5)-Immunocytochemistry-Immunofluorescence-H00051776-M03-img0004.jpg)
![Immunohistochemistry-Paraffin: ZAK Antibody (3G5) [H00051776-M03] - Analysis of monoclonal antibody to ZAK on formalin-fixed paraffin-embedded human prostate. Antibody concentration 3 ug/ml](http://images.novusbio.com/images2/ZAK Antibody (3G5)-Immunohistochemistry-Paraffin-H00051776-M03-img0006.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/images2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Albumin Antibody [NB600-41532] - NLRP12 downregulates multiple JNK activating pathways in hepatocytes. For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/40396), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Albumin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-41532-img0003.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of Bcl-2 in whole cell lysate from Daoy cells. Cells were transfected with (1) scrambled control siRNA or (2) Bcl-2 siRNA. Image from verified customer review.](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0004.jpg)
![Western Blot: Bcl-2 Antibody [NB100-56098] - Analysis of whole cell lysate (WCL) of Jurkat cells using anti-Bcl-2 antibody (NB100-56098) at 1:1000 dilution. HRP conjugated goat anti-rabbit IgG (H+L) cross adsorbed secondary antibody was used with ECL substrate for the detection of Bcl2 antibody bound to the blotted protein. This Bcl2 antibody detected a specific band at expected molecular weight marker position of Bcl2 protein (26kDa).](http://images.novusbio.com/images2/Bcl-2-Antibody-Western-Blot-NB100-56098-img0007.jpg)
![Immunohistochemistry-Paraffin: Bcl-2 Antibody [NB100-56098] - Analysis of a FFPE human breast carcinoma tissue section using 1:1000 dilution of Bcl-2 antibody (NB100-56098) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This antibody generated a diffused cytoplasmic staining of BCL2 in the cancer cells with relatively intense signal in their nuclear membranes. Staining was performed by Histowiz.](http://images.novusbio.com/images2/Bcl-2-Antibody-Immunohistochemistry-Paraffin-NB100-56098-img0011.jpg)
![IL-4 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-4 [Unconjugated]")
![Knockdown Validated: Calreticulin Antibody [NB600-101] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Calreticulin knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1500 of Rabbit Anti-Human Calreticulin Polyclonal Antibody (Catalog # NB600-101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Calreticulin at approximately 55 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/images2/Calreticulin-Antibody-Knockdown-Validated-NB600-101-img0011.jpg)
![Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.](http://images.novusbio.com/images2/Calreticulin-Antibody-Western-Blot-NB600-101-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Calreticulin Antibody [NB600-101] - Immunofluorescence staining of Calreticulin in HCT15 colon cancer cells using NB600-101. Secondary antibody was conjugated with Alexa Fluor 488. Photo courtesy of Dr. Birkenkamp Demtroeder, Arhus University Hospital.](http://images.novusbio.com/images2/Calreticulin-Antibody-Immunocytochemistry-Immunofluorescence-NB600-101-img0005.jpg)
![Western Blot: StAR Antibody [NBP1-33485] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody](http://images.novusbio.com/images2/StAR-Antibody-Western-Blot-NBP1-33485-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: StAR Antibody [NBP1-33485] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: StAR protein stained by StAR antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/StAR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33485-img0005.jpg)
![Immunohistochemistry-Paraffin: StAR Antibody [NBP1-33485] - Human hepatoma. StAR antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/images2/StAR-Antibody-Immunohistochemistry-Paraffin-NBP1-33485-img0004.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0017.jpg)
![Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.](http://images.novusbio.com/images2/CD4-Antibody-Immunohistochemistry-NBP1-19371-img0018.jpg)
![Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.](http://images.novusbio.com/images2/CD4-Antibody-Western-Blot-NBP1-19371-img0002.jpg)
![Western Blot: Ovalbumin Antibody [NB600-922] - Lane 1: Reduced Ovalbumin. Load: 50 ng. Primary antibody: Ovalbumin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Ovalbumin.](http://images.novusbio.com/images2/Ovalbumin-Antibody-Western-Blot-NB600-922-img0015.jpg)
![Western Blot: Ovalbumin Antibody [NB600-922] - Western blot detection of Ovalbumin from hen egg white using NB600-922 at 1:10,000. The blot was blocked ON at 4 degrees C in 3% BSA. The secondary antibody used was a DyLight 649 Conjugated Goat anti-rabbit IgG (H&L) diluted 1:20,000 and incubated 1 hr RT in blocking buffer for fluorescent western blotting.](http://images.novusbio.com/images2/Ovalbumin-Antibody-Western-Blot-NB600-922-img0004.jpg)
![Western Blot: Ovalbumin Antibody [NB600-922] - Analysis using the HRP conjugate of NB600-922. Detection of Lane 1: Ovalbumin (Hen Egg). Load: 50 ng per lane. Dilution: 1:1,000. Predicted/Observed size: 43 kDa, 43 kDa for Ovalbumin. Other band(s): Ovalbumin splice variants and isoforms.](http://images.novusbio.com/images2/Ovalbumin-Antibody-Western-Blot-NB600-922-img0009.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Western Blot analysis with ARNT/HIF-1 beta antibody (H1beta234) [NB100-124], theoretical molecular weight 86.6 kDa. Expression of CD44 mRNA in genetically engineered MDA-MB-231 cells. (A) Immunoblot analysis of HIF-1A or HIF-2A expression in whole cell extracts from MDA-MB-231 cells stably expressing EV, HIF-1A-shRNA or HIF-2A-shRNA under normoxia or in response to 4 h treatment with 200 uM CoCl2. HIF-1B expression was used as a loading control. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0044078) licensed under a CC-BY licence.](http://images.novusbio.com/images2/ARNT-HIF-1-beta-Antibody-(H1beta234)-Western-Blot-NB100-124-img0009.jpg)
![Western Blot: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - HIF was not the only factor stabilizing activated EGFR in VHL-deficient ccRCC cells. A. Western blot analysis of 786-VHL and 786-mock cells stably expressing shRNA constructs. For HIF2A (NB100-480) and ARNT/HIF-1 beta antibody (H1beta234)[NB100-124] analysis, nuclear extracts were generated and analyzed. Anti-HA blot detected HA-VHL. Means and SDs of three separate experiments were shown. Theoretical molecular weight for ARNT/HIF-1 beta is 86.6 kDa, observed molecular weight ~90 kDa. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0023936) licensed under a CC-BY licence.](http://images.novusbio.com/images2/ARNT-HIF-1-beta-Antibody-(H1beta234)-Western-Blot-NB100-124-img0010.jpg)
![Immunohistochemistry: ARNT/HIF-1 beta Antibody (H1beta234) [NB100-124] - Staining of human glioblastoma multi-forme utilizing ARNT/HIF-1 beta antibody (H1beta234) [NB100-124].](http://images.novusbio.com/images2/ARNT-HIF-1-beta-Antibody-(H1beta234)-Immunohistochemistry-NB100-124-img0005.jpg)
![Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Western-Blot-NBP2-34260-img0006.jpg)
![Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-NBP2-34260-img0005.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/images2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: AHR Antibody [NB100-128] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti AHR [NB100-128] at a 1:100 dilution overnight at 4C and detected with an anti-goat DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/AHR-Antibody-Immunocytochemistry-Immunofluorescence-NB100-128-img0004.jpg)
![Immunohistochemistry-Paraffin: AHR Antibody [NB100-128] - Analysis of FFPE human liver using 1:200 conc. of AHR antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/images2/AHR-Antibody-Immunohistochemistry-Paraffin-NB100-128-img0006.jpg)
![Gel Super Shift Assays: AHR Antibody [NB100-128] - EMSA validation of AHR antibody on U937 cells nuclear protein. Image submitted by verified customer - see review for protocol details.](http://images.novusbio.com/images2/AHR-Antibody-Gel-Super-Shift-Assays-NB100-128-img0003.jpg)
![Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by chemiluminescence.](http://images.novusbio.com/images2/POMC-Antibody-Western-Blot-NB100-1533-img0005.jpg)
![Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows point to perinuclear POMC. KDEL marks the ER. Representative data from at least 2 independent experiments are shown. Image collected and cropped by CiteAb from the following publication (jci.org/articles/view/96420), licensed under a CC-BY licence.](http://images.novusbio.com/images2/POMC-Antibody-Immunohistochemistry-NB100-1533-img0008.jpg)
![Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/images2/POMC-Antibody-Flow-Cytometry-NB100-1533-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")